GEO series

RNA-seq and ChIP-seq analysis of H3K27ac, H3K27me3, H3K9me2, and JMJD1A in 3T3-L1 cells and ChIP-seq analysis of NFIC in imSVF cells.

GSE266317 Mus musculus Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing 14 samples Submitted 2025/07/20 Platform GPL21273Platform GPL24247Platform GPL9185

Summary

Adipose tissue remodels via hypertrophy or hyperplasia in response to nutrient status, but the mechanisms governing these expansion modes remain unclear. Here, we identify a nutrient-sensitive epigenetic circuit linking glucose metabolism to chromatin remodeling during adipogenesis. Upon glucose stimulation, α-ketoglutarate (α-KG) accumulates in the nucleus and activates the histone demethylase JMJD1A to remove repressive H3K9me2 marks at glycolytic and adipogenic gene loci, including Pparg. JMJD1A is recruited to premarked promoter chromatin via NFIC, enabling carbohydrate-responsive element-binding protein (ChREBP) binding and transcriptional activation. This feedforward mechanism couples nutrient flux to chromatin accessibility and gene expression. In vivo, JMJD1A is essential for de novo adipogenesis and hyperplastic expansion in visceral fat under nutrient excess. JMJD1A deficiency impairs hyperplasia, exacerbates adipocyte hypertrophy, and induces local inflammation. These findings define a glucose–α-KG–JMJD1A–ChREBP axis regulating depot-specific adipogenesis and uncover a chromatin-based mechanism by which glucose metabolism governs adaptive adipose tissue remodeling.

Published in

Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling

Yang C, Arai M, Ariyanto EF et al. · Cell reports 2025 · PMID 40720241 · doi:10.1016/j.celrep.2025.116060

This dataset

Open GSE266317 in finder → Find more mouse RNA-seq datasets →

Download

Direct links to NCBI, no account and no request form: the whole study as GSE266317_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 14 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1106622 and SRA study SRP505074. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40720241) ↗

Study design

14 conditions, each sampled once — no replicated groups

Read from 14 sample titles: 14 distinct titles with little repetition. Check it against the sample list below before relying on it.

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.