GEO series
Immune hallmarks of recurrent immune checkpoint inhibitor-mediated inflammatory arthritis
GSE338460
Homo sapiens
Expression profiling by high throughput sequencing; Other
25 samples
Submitted 2026/07/25
Platform GPL24676
Summary
Immune checkpoint inhibitor (ICI) therapy is often associated with immune-related adverse events including inflammatory arthritis (ICI-IA). However, the mechanisms underlying ICI-IA, especially its recurrence, have not been fully investigated. The purpose of this study is to elucidate mechanisms of recurrent ICI-IA by analyzing longitudinal synovial fluid (SF) samples. SF samples were collected from six ICI-IA patients at the first and second occurrences of ICI-IA and analyzed with single-cell RNA sequencing (scRNAseq) (n=3), scTCRseq (n=3), scBCRseq (n=3), and flow cytometry (n=6). SF samples from cancer-naïve osteoarthritis patients (n=6) were used as negative controls. Analysis revealed that effector CD8+ T cells and PD-1hi CXCL13hi CD4+ T cells were enriched in the SF of ICI-IA patients. Ninety three percent and fifty percent of the top ten expanded clones of effector CD8+ T cells and PD-1hi CXCL13hi CD4+ T cells, respectively, were shared between the first and second ICI-IA flare. These top clones were characterized by the production of pro-inflammatory type 1 cytokines including IFNg, TNFa, and IL-21, especially in the second flare, suggesting immune memory responses to cognate antigen. Cell-cell communication analysis suggests that effector CD8+ T cells and PD-1hi CXCL13hi CD4+ T cells interacted with each other and with myeloid cells and B cells through chemokines (CXCL9/10/11/13, CCL3) and cytokines (MIF, IL-2/7/15/21). Overall, longitudinal SF analysis from ICI-IA patients for the first time revealed the expansion of effector CD8+ T cells and PD-1hi CXCL13hi CD4+ T cells with type 1 cytokine signatures that potentially contribute to development or recurrence of ICI-IA.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE338460_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 25 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1494521 and SRA study SRP717645. Searching any of these in the dataset finder brings you back here.
Study design
25 conditions, each sampled once — no replicated groups
Read from 25 sample titles: 25 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9874759 Patient1_First ICI-IA_GEX
- GSM9874760 Patient1_Second ICI-IA_GEX
- GSM9874761 Patient2_First ICI-IA_GEX
- GSM9874762 Patient2_Second ICI-IA_GEX
- GSM9874763 Patient3_First ICI-IA_GEX
- GSM9874764 Patient3_Second ICI-IA_GEX
- GSM9874765 Patient4_OA_GEX
- GSM9874766 Patient5_OA_GEX
- GSM9874767 Patient6_OA_GEX
- GSM9874768 Patient1_First ICI-IA_TCR
- GSM9874769 Patient1_Second ICI-IA_TCR
- GSM9874770 Patient2_First ICI-IA_TCR
- GSM9874771 Patient2_Second ICI-IA_TCR
- GSM9874772 Patient3_First ICI-IA_TCR
- GSM9874773 Patient3_Second ICI-IA_TCR
- GSM9874774 Patient4_OA_TCR
- GSM9874775 Patient5_OA_TCR
- GSM9874776 Patient6_OA_TCR
- GSM9874777 Patient2_First ICI-IA_BCR
- GSM9874778 Patient2_Second ICI-IA_BCR
- GSM9874779 Patient3_First ICI-IA_BCR
- GSM9874780 Patient3_Second ICI-IA_BCR
- GSM9874781 Patient4_OA_BCR
- GSM9874782 Patient5_OA_BCR
- GSM9874783 Patient6_OA_BCR
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