GEO series
Recruitment of plasma cells from follicular and extrafollicular immune reactions to the bone marrow I
GSE252959
Homo sapiens
Expression profiling by high throughput sequencing; Other
152 samples
Submitted 2024/04/16
Platform GPL30173
Summary
Bone marrow plasma cells (BMPC) are the correlate of humoral immunity, consistently releasing antibodies into the bloodstream. It remains unclear if BMPC reflect different activation environments or maturation of their precursors. Here we define human BMPC heterogeneity and track the recruitment of antibody-secreting cells (ASC) from SARS-CoV-2 vaccine immune reactions to the bone marrow (BM). Trajectories based on single-cell transcriptomes and repertoires of peripheral and BM ASC reveal sequential colonisation of BMPC compartments. In activated B cells, IL-21 suppresses CD19 expression, indicating that CD19low-BMPC are derived from follicular, while CD19high-BMPC originate from extrafollicular immune reactions. In primary immune reactions, both CD19low- and CD19high-BMPC compartments are populated. In secondary immune reactions, most BMPC are recruited to CD19high-BMPC compartments, reflecting their origin from extrafollicular reactivations of memory B cells. A pattern also observed in vaccinated-convalescent individuals and upon diphtheria/tetanus/pertussis recall-vaccination. Thus, BMPC diversity reflects the evolution of a given humoral immune response.
Published in
Ferreira-Gomes M, Chen Y, Durek P et al.
· Nature communications 2024
· PMID 38755157
· doi:10.1038/s41467-024-48570-0
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE252959_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 152 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1063318 and SRA study SRP482991. Searching any of these in the dataset finder brings you back here.
Study design
40 conditions, each sampled once — no replicated groups
Read from the first 40 of 152 sample titles: 40 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
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- GSM8010964 GEX-Seq, BNT d14, donor 11, 12, 14 and 15
- GSM8010965 CITE-Seq, BNT d14, donor 11, 12, 14 and 15
- GSM8010966 BCR-Seq, BNT d14, donor 11, 12, 14 and 15
- GSM8010967 TCR-Seq, BNT d14, donor 11, 12, 14 and 15
- GSM8010968 GEX-Seq, BNT d14, donor 13, 16 and 17
- GSM8010969 CITE-Seq, BNT d14, donor 13, 16 and 17
- GSM8010970 BCR-Seq, BNT d14, donor 13, 16 and 17
- GSM8010971 TCR-Seq, BNT d14, donor 13, 16 and 17
- GSM8010972 GEX-Seq, BNT/BNT d7, donor 12, 13, 15 and 16
- GSM8010973 CITE-Seq, BNT/BNT d7, donor 12, 13, 15 and 16
- GSM8010974 BCR-Seq, BNT/BNT d7, donor 12, 13, 15 and 16
- GSM8010975 TCR-Seq, BNT/BNT d7, donor 12, 13, 15 and 16
- GSM8010976 GEX-Seq, BNT d7, donor 11, 12 and 13
- GSM8010977 CITE-Seq, BNT d7, donor 11, 12 and 13
- GSM8010978 BCR-Seq, BNT d7, donor 11, 12 and 13
- GSM8010979 TCR-Seq, BNT d7, donor 11, 12 and 13
- GSM8010980 GEX-Seq, BNT d7, donor 14, 15, 16 and 17
- GSM8010981 CITE-Seq, BNT d7, donor 14, 15, 16 and 17
- GSM8010982 BCR-Seq, BNT d7, donor 14, 15, 16 and 17
- GSM8010983 TCR-Seq, BNT d7, donor 14, 15, 16 and 17
- GSM8010984 GEX-Seq, BNT d14, donor 21, 22, 23 and 24
- GSM8010985 CITE-Seq, BNT d14, donor 21, 22, 23 and 24
- GSM8010986 BCR-Seq, BNT d14, donor 21, 22, 23 and 24
- GSM8010987 TCR-Seq, BNT d14, donor 21, 22, 23 and 24
- GSM8010988 GEX-Seq, BNT d14, donor 25, 26, 27 and 28
- GSM8010989 CITE-Seq, BNT d14, donor 25, 26, 27 and 28
- GSM8010990 BCR-Seq, BNT d14, donor 25, 26, 27 and 28
- GSM8010991 TCR-Seq, BNT d14, donor 25, 26, 27 and 28
- GSM8010992 GEX-Seq, BNT/BNT d7, donor 21, 22, 23 and 24
- GSM8010993 CITE-Seq, BNT/BNT d7, donor 21, 22, 23 and 24
- GSM8010994 BCR-Seq, BNT/BNT d7, donor 21, 22, 23 and 24
- GSM8010995 TCR-Seq, BNT/BNT d7, donor 21, 22, 23 and 24
- GSM8010996 GEX-Seq, BNT/BNT d7, donor 25, 26, 27 and 28
- GSM8010997 CITE-Seq, BNT/BNT d7, donor 25, 26, 27 and 28
- GSM8010998 BCR-Seq, BNT/BNT d7, donor 25, 26, 27 and 28
- GSM8010999 TCR-Seq, BNT/BNT d7, donor 25, 26, 27 and 28
- GSM8011000 GEX-Seq, BNT d7, donor 21, 22, 23 and 24
- GSM8011001 CITE-Seq, BNT d7, donor 21, 22, 23 and 24
- GSM8011002 BCR-Seq, BNT d7, donor 21, 22, 23 and 24
- GSM8011003 TCR-Seq, BNT d7, donor 21, 22, 23 and 24
+ 112 more — browse all 152 samples with per-sample file links →
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