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Divergent spatial microdomains drive inflammation and repair in Ulcerative and Immune Checkpoint Therapy Colitis - Spatial Transcriptomics

GSE189184 Homo sapiens Expression profiling by high throughput sequencing; Other 16 samples Submitted 2024/04/12 Platform GPL18573
Summary
Adult inflammatory bowel disease is incompletely understood. We combine unbiased single-cell RNA sequencing (gene expression profiling, CITE-seq derived cell surface protein data, TCR and BCR sequence data) with unbiased spatial transcriptomics to interrogate changes across immune and non-immune populations in colitis and health, across tissue and blood. We compare idiopathic ulcerative colitis with hitherto under-studied immune checkpoint therapy induced colitis, utilizing non-inflamed disease states as additional controls. We identify patterns of inflammation and response unique and common to both diseases, and infer changes in cell trafficking with potential therapeutic implications. We go on to localize disease-specific changes in tissue using spatial transcriptomics, leveraging this data to interrogate cellular interactions in an unbiased manner, allowing us to describe novel microdomains of inflammation and repair.
Published in
Tracking in situ checkpoint inhibitor-bound target T cells in patients with checkpoint-induced colitis
Gupta T, Antanaviciute A, Hyun-Jung Lee C et al. · Cancer cell 2024 · PMID 38744246 · doi:10.1016/j.ccell.2024.04.010
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Direct links to NCBI, no account and no request form: the whole study as GSE189184_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 16 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA781903 and SRA study SRP346915. Searching any of these in the dataset finder brings you back here.

Study design
6 × GI vs 3 × PRISE vs 2 × GI 6967_B

Supports a between-group comparison across 11 samples.

3 replicated groups read from 16 sample titles; they account for 11 of them. Check it against the sample list below before relying on it.

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