GEO series
Single-cell and spatial transcriptomics analysis of tobacco-associated lung adenocarcinoma development, and in response to targeting inflammation alone or in combination with immune checkpoint blockade in precancerous phase [scRNA-seq]
GSE300288
Mus musculus
Expression profiling by high throughput sequencing
31 samples
Submitted 2025/10/11
Platform GPL24247
Summary
Understanding cellular processes underlying early lung adenocarcinoma (LUAD) development is needed to devise intervention strategies. Here, we performed single-cell RNA sequencing (scRNA-seq) of mouse lungs from Gprc5a−/− mice during lung tumor development and also following therapeutic targeting of inflammation (IL-1 ) alone and in combination with immune checkpoint blockade in precancerous stages. We coupled scRNA-seq analysis with spatial transcriptomics of mouse lungs under different conditions.
Published in
Peng F, Sinjab A, Dai Y et al.
· Cancer cell 2026
· PMID 41202811
· doi:10.1016/j.ccell.2025.10.004
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE300288_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 31 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1280046 and SRA study SRP593376. Searching any of these in the dataset finder brings you back here.
Study design
8 conditions, mostly with about 4 replicates each
Lungs of Gprc5a-/- mice exposed to Control Ig… ×4
Lungs of Gprc5a-/- mice exposed to anti-PD-1 … ×4
Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽… ×4
Lungs of Gprc5a-/- mice exposed to Control Ig… ×4
Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽… ×4
Lungs of Gprc5a-/- mice exposed to anti-PD-1 … ×4
Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽… ×4
Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽… ×3
Supports a between-group comparison across 31 samples.
8 replicated groups read from 31 sample titles; they account for 31 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9057709 Lungs of Gprc5a-/- mice exposed to Control IgG and analyzed at 3 months post-NNK, replicate 1
- GSM9057710 Lungs of Gprc5a-/- mice exposed to anti-PD-1 and analyzed at 3 months post-NNK, replicate 1
- GSM9057711 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and anti-PD-1 and analyzed at 3 months post-NNK, replicate 1
- GSM9057712 Lungs of Gprc5a-/- mice exposed to Control IgG and analyzed at 3 months post-NNK, replicate 2
- GSM9057713 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and analyzed at 3 months post-NNK, replicate 2
- GSM9057714 Lungs of Gprc5a-/- mice exposed to anti-PD-1 and analyzed at 3 months post-NNK, replicate 2
- GSM9057715 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and anti-PD-1 and analyzed at 3 months post-NNK, replicate 2
- GSM9057716 Lungs of Gprc5a-/- mice exposed to Control IgG and analyzed at 3 months post-NNK, replicate 3
- GSM9057717 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and analyzed at 3 months post-NNK, replicate 3
- GSM9057718 Lungs of Gprc5a-/- mice exposed to anti-PD-1 and analyzed at 3 months post-NNK, replicate 3
- GSM9057719 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and anti-PD-1 and analyzed at 3 months post-NNK, replicate 3
- GSM9057720 Lungs of Gprc5a-/- mice exposed to Control IgG and analyzed at 3 months post-NNK, replicate 4
- GSM9057721 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and analyzed at 3 months post-NNK, replicate 4
- GSM9057722 Lungs of Gprc5a-/- mice exposed to anti-PD-1 and analyzed at 3 months post-NNK, replicate 4
- GSM9057723 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and anti-PD-1 and analyzed at 3 months post-NNK, replicate 4
- GSM9057724 Lungs of Gprc5a-/- mice exposed to Control IgG and analyzed at 7 months post-NNK, replicate 1
- GSM9057725 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and analyzed at 7 months post-NNK, replicate 1
- GSM9057726 Lungs of Gprc5a-/- mice exposed to anti-PD-1 and analyzed at 7 months post-NNK, replicate 1
- GSM9057727 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and anti-PD-1 and analyzed at 7 months post-NNK, replicate 1
- GSM9057728 Lungs of Gprc5a-/- mice exposed to Control IgG and analyzed at 7 months post-NNK, replicate 2
- GSM9057729 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and analyzed at 7 months post-NNK, replicate 2
- GSM9057730 Lungs of Gprc5a-/- mice exposed to anti-PD-1 and analyzed at 7 months post-NNK, replicate 2
- GSM9057731 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and anti-PD-1 and analyzed at 7 months post-NNK, replicate 2
- GSM9057732 Lungs of Gprc5a-/- mice exposed to Control IgG and analyzed at 7 months post-NNK, replicate 3
- GSM9057733 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and analyzed at 7 months post-NNK, replicate 3
- GSM9057734 Lungs of Gprc5a-/- mice exposed to anti-PD-1 and analyzed at 7 months post-NNK, replicate 3
- GSM9057735 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and anti-PD-1 and analyzed at 7 months post-NNK, replicate 3
- GSM9057736 Lungs of Gprc5a-/- mice exposed to Control IgG and analyzed at 7 months post-NNK, replicate 4
- GSM9057737 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and analyzed at 7 months post-NNK, replicate 4
- GSM9057738 Lungs of Gprc5a-/- mice exposed to anti-PD-1 and analyzed at 7 months post-NNK, replicate 4
- GSM9057739 Lungs of Gprc5a-/- mice exposed to anti-IL-1𝛽 and anti-PD-1 and analyzed at 7 months post-NNK, replicate 4
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