GEO series
MRPGRX2 Antagonist Treatment Prevents Inflammation and Disease in a Mouse Model of Atopic Dermatitis Dataset 2
GSE293315
Mus musculus
Expression profiling by high throughput sequencing
35 samples
Submitted 2026/04/01
Platform GPL21493
Summary
The Mas-Related G-Protein Coupled Receptor X2 (MRGPRX2) is a promiscuous receptor expressed on mast cells that mediates IgE-independent mast cell activation and degranulation in response to a variety of diverse agonists and has been implicated in numerous inflammatory conditions, including atopic dermatitis. We investigated if the small molecule MRGPRX2 antagonist, INCB000262, improves disease phenotype in the house dust mite + Staphylococcus aureus enterotoxin type B (HDM+SEB) mouse model of atopic dermatitis and further characterized mast cell-mediated aspects of disease. MRGPRX2 knock-in mice were sensitized to HDM+SEB and orally dosed once daily with either vehicle or INCB000262 for 4 weeks, and efficacy and skin transcriptional changes evaluated. INCB000262 attenuated HDM+SEB-induced atopic dermatitis in vivo with improvements observed in skin thickness, transepidermal water loss, tissue weight, and overall clinical score. In addition, transcriptome changes induced by HDM+SEB exhibited a mast cell activation signature consistent with human atopic dermatitis lesional skin, which was attenuated by antagonist treatment. Overall, the MRGPRX2 antagonist, INCB000262, attenuated HDM+SEB-induced atopic dermatitis disease phenotypes and mast cell-associated transcriptional changes, supporting further evaluation of INCB000262 as an oral treatment for atopic dermatitis and other mast cell-mediated diseases.
Published in
MRGPRX2 antagonist treatment prevents inflammation and disease in a mouse model of atopic dermatitis
Wollam J, Solomon M, Villescaz C et al.
· The Journal of investigative dermatology 2026
· PMID 42092534
· doi:10.1016/j.jid.2026.04.021
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE293315_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 35 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1244004 and SRA study SRP575111. Searching any of these in the dataset finder brings you back here.
Study design
13 × skin, biopsy, HDM+SEB, INCB000262, animal vs 12 × skin, biopsy, vehicle, vehicle, animal vs 10 × skin, biopsy, HDM+SEB, vehicle, animal
Supports a between-group comparison across 35 samples.
3 replicated groups read from 35 sample titles; they account for 35 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM8880197 skin, biopsy, vehicle, vehicle, animal 1
- GSM8880198 skin, biopsy, vehicle, vehicle, animal 2
- GSM8880199 skin, biopsy, vehicle, vehicle, animal 3
- GSM8880200 skin, biopsy, vehicle, vehicle, animal 4
- GSM8880201 skin, biopsy, vehicle, vehicle, animal 5
- GSM8880202 skin, biopsy, vehicle, vehicle, animal 6
- GSM8880203 skin, biopsy, vehicle, vehicle, animal 7
- GSM8880204 skin, biopsy, vehicle, vehicle, animal 8
- GSM8880205 skin, biopsy, vehicle, vehicle, animal 9
- GSM8880206 skin, biopsy, vehicle, vehicle, animal 10
- GSM8880207 skin, biopsy, vehicle, vehicle, animal 11
- GSM8880208 skin, biopsy, vehicle, vehicle, animal 12
- GSM8880209 skin, biopsy, HDM+SEB, vehicle, animal 49
- GSM8880210 skin, biopsy, HDM+SEB, vehicle, animal 50
- GSM8880211 skin, biopsy, HDM+SEB, vehicle, animal 51
- GSM8880212 skin, biopsy, HDM+SEB, vehicle, animal 52
- GSM8880213 skin, biopsy, HDM+SEB, vehicle, animal 53
- GSM8880214 skin, biopsy, HDM+SEB, vehicle, animal 54
- GSM8880215 skin, biopsy, HDM+SEB, vehicle, animal 55
- GSM8880216 skin, biopsy, HDM+SEB, vehicle, animal 57
- GSM8880217 skin, biopsy, HDM+SEB, vehicle, animal 58
- GSM8880218 skin, biopsy, HDM+SEB, vehicle, animal 59
- GSM8880219 skin, biopsy, HDM+SEB, INCB000262, animal 60
- GSM8880220 skin, biopsy, HDM+SEB, INCB000262, animal 61
- GSM8880221 skin, biopsy, HDM+SEB, INCB000262, animal 62
- GSM8880222 skin, biopsy, HDM+SEB, INCB000262, animal 63
- GSM8880223 skin, biopsy, HDM+SEB, INCB000262, animal 64
- GSM8880224 skin, biopsy, HDM+SEB, INCB000262, animal 65
- GSM8880225 skin, biopsy, HDM+SEB, INCB000262, animal 66
- GSM8880226 skin, biopsy, HDM+SEB, INCB000262, animal 67
- GSM8880227 skin, biopsy, HDM+SEB, INCB000262, animal 68
- GSM8880228 skin, biopsy, HDM+SEB, INCB000262, animal 69
- GSM8880229 skin, biopsy, HDM+SEB, INCB000262, animal 70
- GSM8880230 skin, biopsy, HDM+SEB, INCB000262, animal 71
- GSM8880231 skin, biopsy, HDM+SEB, INCB000262, animal 72
Similar datasets
- GSE228960 Bulk RNA-Seq from kidneys of uninephrectomized db/db mice as a model of severe diabetic kidney disease 28 samples
- GSE319035 P21+TREM2+-Senescent Macrophages Fuel Inflammaging and Metabolic Dysfunction-Associated Steatotic Liver Disease [NAFLD_time_course] 77 samples
- GSE319769 Transcriptomic profiling of the intra-amygdala kainate mouse model across the stages of epileptogenesis 36 samples
- GSE300288 Single-cell and spatial transcriptomics analysis of tobacco-associated lung adenocarcinoma development, and in response to targeting inflammation alone or in combination with immune checkpoint blockade in precancerous phase [scRNA-seq] 31 samples
- GSE343043 Disease context dictates the cellular targets of IL-17 in inflammatory skin disease 29 samples
- GSE341985 Diseaase context dictates the cellular targets of IL-17 in inflammatory skin disease [2] 27 samples
- GSE271662 RNA-seq of Mouse Spinal Cord after Lateral Compression Spinal Cord Injury followed by Delayed Atorvastatin or Vehicle Treatment [Bulk RNA-seq of mice spinal cords] 16 samples
- GSE328496 Gene expression + ATAC profiling of trisomic hippocampal neurons upon SAHA treatment [RNA-seq] 16 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.