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Single-cell transcriptomic profiling unveils tumor-mediated reprogramming of neutrophils and their unique vulnerability that inhibits metastasis

GSE327176 Mus musculus Expression profiling by high throughput sequencing 25 samples Submitted 2026/07/17 Platform GPL24247
Summary
We performed single cell RNA sequencing (scRNAseq) with the 10x Genomics platform to study how neutrophils are reprogrammed during breast tumorigenesis. Neutrophils acquire a pro-metastatic phenotype induced by signals eminating from the tumor. This comprehensive scRNAseq study assessed neutrophils from various tissues in naive and tumor-bearing MMTV-PyMT mice to uncover how neutrophils are transcriptionally modified. We found that neutrophils are reprogrammed similarly amongst tissues in tumor-bearing mice, and that G-CSF is the main driver of this.
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Direct links to NCBI, no account and no request form: the whole study as GSE327176_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 25 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1449819 and SRA study SRP689778. Searching any of these in the dataset finder brings you back here.

Study design
8 conditions, mostly in duplicate
Wildtype_BM ×2 NT_BM ×2 GCSF_BM ×2 Wildtype_Blood ×2 MMTV_Pymt_Blood ×2 Wildtype_Lung ×2 MMTV_Pymt_Lung ×2 MMTV_Pymt_Pri_Tumor ×2 +9 more

Supports a case/control comparison: 2 samples read as cases, 7 as controls.

8 replicated groups read from 25 sample titles; they account for 16 of them. Check it against the sample list below before relying on it.

Samples in this study
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