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Transcriptomic profiling of pancreatic stellate cells and pancreatic cancer cells in a gemcitabine-driven tumor-stroma supernatant transfer model

GSE324779 Mus musculus Expression profiling by high throughput sequencing 12 samples Submitted 2026/03/18 Platform GPL34290
Summary
Pancreatic ductal adenocarcinoma (PDAC) is characterized by extensive tumor-stroma interactions that influence disease progression and therapy response. To investigate transcriptional changes associated with chemotherapy-induced tumor-stroma signaling, RNA sequencing was performed in a supernatant transfer system. Murine pancreatic cancer cells (KPC4) were treated with gemcitabine, and supernatants from gemcitabine-treated or vehicle-treated tumor cells were transferred to murine pancreatic stellate cells (PSC4). In a second experiment, supernatants from PSC4 cells previously exposed to tumor cell supernatants were transferred back to pancreatic cancer cells. RNA sequencing was performed to characterize gene expression changes associated with chemotherapy-induced tumor-stroma communication.
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Direct links to NCBI, no account and no request form: the whole study as GSE324779_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 12 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1435881 and SRA study SRP682957. Searching any of these in the dataset finder brings you back here.

Study design
3 × PSC4 cells treated with Control-SU, vs 3 × PSC4 cells treated with Gem-SU, vs 3 × KPC4 cells treated with Control-PSC-SU, vs 3 × KPC4 cells treated with Gem-PSC-SU,

Supports a between-group comparison across 12 samples.

4 replicated groups read from 12 sample titles; they account for 12 of them. Check it against the sample list below before relying on it.

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