GEO series
Time-resolved transcriptomic profiling of surgical wounds identifies stage-specific therapeutic targets for residual ovarian cancer
GSE325548
Mus musculus
Expression profiling by high throughput sequencing
48 samples
Submitted 2026/03/27
Platform GPL9185
Summary
Background: The optimal timing of adjuvant chemotherapy after cytoreductive surgery in epithelial ovarian cancer remains uncertain, and perioperative wound-healing responses may transiently create a pro-tumorigenic and drug-resistant microenvironment. This study aimed to characterize time-dependent wound-induced transcriptomic alterations and to identify pharmacologic agents capable of reversing these responses. Methods: An ID8 murine ovarian cancer model was used to compare no treatment, anesthesia alone, and anesthesia plus surgical wounding mimicking futile laparotomy. Tumors were collected at baseline, 1 day (T1), 1 week (T2), and 2 weeks (T3) after intervention. RNA sequencing was performed, and wound-specific differentially expressed genes (WsDEGs) were defined by excluding anesthesia- and progression-related signatures. Functional enrichment analyses were conducted, followed by transcriptome-based drug repurposing using the REMEDY platform to identify compounds predicted to reverse wound-induced gene expression profiles. Results: Surgical wounding significantly increased tumor burden at T1. Transcriptomic analyses revealed distinct, time-dependent wound-associated programs. At T1, WsDEGs were enriched in inflammatory signaling, coagulation, angiogenesis, and immune cell migration, with Vorinostat and Homohar-ringtonine identified as top candidates to counteract these signatures. At T2, pathways related to cell survival, adhesion, and morphogenesis predominated, with LY-2090314, Artesunate, and Birinapant emerging as potential modulators. At T3, cell-cycle regulation and lipid metabolic pathways were dominant, and Fulvestrant, Atorvastatin, Imatinib, and ABT-737 were predicted to inhibit these processes. Conclusions: Perioperative sur-gical wounding induces dynamic, stage-specific transcriptomic programs that may promote ovarian cancer progression and alter drug responsiveness. These findings support time-adapted perioperative pharmacologic strategies to optimize postoperative cancer therapy.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE325548_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 48 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1440454 and SRA study SRP685464. Searching any of these in the dataset finder brings you back here.
Study design
40 conditions, each sampled once — no replicated groups
Read from the first 40 of 48 sample titles: 40 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9608249 ID8 cells, Base1, Day0
- GSM9608250 ID8 cells, Base10, Day0
- GSM9608251 ID8 cells, Base3, Day0
- GSM9608252 ID8 cells, Base5, Day0
- GSM9608253 ID8 cells, Base9, Day0
- GSM9608254 ID8 cells, T1_N2, Day1
- GSM9608255 ID8 cells, T1_N4, Day1
- GSM9608256 ID8 cells, T1_N5, Day1
- GSM9608257 ID8 cells, T1_A2, Day1
- GSM9608258 ID8 cells, T1_A3, Day1
- GSM9608259 ID8 cells, T1_AW1, Day1
- GSM9608260 ID8 cells, T1_AW3, Day1
- GSM9608261 ID8 cells, T1_AW5, Day1
- GSM9608262 ID8 cells, T1_AW6, Day1
- GSM9608263 ID8 cells, T2_N1, Day7
- GSM9608264 ID8 cells, T2_N3, Day7
- GSM9608265 ID8 cells, T2_N4, Day7
- GSM9608266 ID8 cells, T2_N5, Day7
- GSM9608267 ID8 cells, T2_N8, Day7
- GSM9608268 ID8 cells, T2_A1, Day7
- GSM9608269 ID8 cells, T2_A10, Day7
- GSM9608270 ID8 cells, T2_A2, Day7
- GSM9608271 ID8 cells, T2_A3, Day7
- GSM9608272 ID8 cells, T2_A4, Day7
- GSM9608273 ID8 cells, T2_A5, Day7
- GSM9608274 ID8 cells, T2_A6, Day7
- GSM9608275 ID8 cells, T2_A7, Day7
- GSM9608276 ID8 cells, T2_A8, Day7
- GSM9608277 ID8 cells, T2_AW2, Day7
- GSM9608278 ID8 cells, T2_AW3, Day7
- GSM9608279 ID8 cells, T2_AW4, Day7
- GSM9608280 ID8 cells, T2_AW6, Day7
- GSM9608281 ID8 cells, T2_AW8, Day7
- GSM9608282 ID8 cells, T2_AW9, Day7
- GSM9608283 ID8 cells, T3_N1, Day14
- GSM9608284 ID8 cells, T3_N3, Day14
- GSM9608285 ID8 cells, T3_N4, Day14
- GSM9608286 ID8 cells, T3_N5, Day14
- GSM9608287 ID8 cells, T3_N6, Day14
- GSM9608288 ID8 cells, T3_A1, Day14
+ 8 more — browse all 48 samples with per-sample file links →
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