GEO series

scRNA-seq of mouse cochlea organoid cells

GSE326100 Mus musculus Expression profiling by high throughput sequencing 768 samples Submitted 2026/04/07 Platform GPL19057

Summary

Neonatal mouse cochlear epithelium retains regenerative capacity, attributed to the greater epithelial ridge (GER) cells. GER cells proliferate to form organoids and give rise to nascent hair cells and supporting cells. To investigate the growth-promoting pathways in GER cells, we generated organoids from highly enriched mouse GER cells and performed single-cell RNA sequencing. Data analysis revealed changes in gene expression in GER-derived cells during the early growth phase of organoid development. Integrating single-cell RNA sequencing data with in vitro and in vivo functional assays, we identified galectins and Myc activity as essential regulators of GER cell proliferation.

Published in

Galectin and Myc enable cochlear progenitor expansion in vitro and in vivo

Kubota M, Abitbol JM, Lee PK et al. · bioRxiv : the preprint server for biology 2026 · PMID 42282633 · doi:10.64898/2026.06.03.729765

This dataset

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Download

Direct links to NCBI, no account and no request form: the whole study as GSE326100_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 768 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1443350 and SRA study SRP686985. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42282633) ↗

Study design

40 × Day7.organoid.Rep1_Cell

Supports a between-group comparison across 40 samples.

1 replicated groups read from the first 40 of 768 sample titles; they account for 40 of them. Check it against the sample list below before relying on it.

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