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Bulk RNA-seq of mouse brain following closed head injury: role of IL-1R1 signaling

GSE326452 Mus musculus Expression profiling by high throughput sequencing 200 samples Submitted 2026/07/29 Platform GPL34290
Summary
Bulk RNA-seq was performed on brain tissue from mice following closed head injury (CHI) or sham surgery at 9h, 24h, and 72h post-injury. Four genotypes were studied: C57BL/6J wild-type, IL-1R1 global knockout (gKO), IL-1R1 endothelial restore (eRestore), and IL-1R1 neuronal restore (nRestore) to dissect cell-type specific IL-1R1 signaling contributions to the transcriptomic response to traumatic brain injury.
Published in
A single mild closed-head injury disrupts synaptic strength and promotes hippocampal hyperexcitability in mice
Vincent JC, Lanning MJ, Saito K et al. · Brain communications 2026 · PMID 42488755 · doi:10.1093/braincomms/fcag268
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE326452_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 200 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1445297 and SRA study SRP687867. Searching any of these in the dataset finder brings you back here.

Study design
8 conditions, mostly in triplicate
C57BL/6J_F_CHI_24h_A ×8 C57BL/6J_F_CHI_9h_A ×7 C57BL/6J_F_CHI_72h_A ×6 C57BL/6J_M_CHI_24h_A ×6 C57BL/6J_M_CHI_72h_A ×4 C57BL/6J_F_sham_24h_A ×3 C57BL/6J_F_sham_72h_A ×3 C57BL/6J_F_sham_9h_A ×3

Supports a between-group comparison across 40 samples.

8 replicated groups read from the first 40 of 200 sample titles; they account for 40 of them. Check it against the sample list below before relying on it.

Samples in this study

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