GEO series
Astrocyte glucocorticoid receptor signaling restricts neuronal plasticity [RNA-seq]
GSE306262
Mus musculus
Expression profiling by high throughput sequencing
40 samples
Submitted 2026/03/20
Platform GPL19057
Summary
Sensory experience refines neural circuits during critical periods of postnatal development. Although neuronal activity is known to orchestrate the circuit wiring that underlies this process, the environmental cues that restrain developmental plasticity as animals mature are less clear. Here, we examine the experience-dependent maturation of the mouse primary visual cortex (V1) across postnatal development using paired single-cell transcriptomic and chromatin accessibility sequencing. In addition to identifying the activity-dependent gene programs that emerge within each cortical cell type, we find that light exposure drives astrocyte maturation through cell type-specific recruitment of the glucocorticoid receptor (Nr3c1/GR) to chromatin. Astrocyte GR signaling activates an extensive gene regulatory program that is partially conserved in human brain development and promotes maturation processes that may trigger critical period closure. Collectively, these findings reveal that astrocyte GR signaling restricts neuronal plasticity. Glucocorticoid regulation of astrocyte maturation may also contribute to the effects of early-life stress across the brain, and the disruption of this process may increase susceptibility to neuropsychiatric disease.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE306262_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 40 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1310321 and SRA study SRP612553. Searching any of these in the dataset finder brings you back here.
Study design
12 conditions, mostly in triplicate
RNA-seq on P21 DR V1 astrocyte GR-Con nuclei, ×4
RNA-seq on P21 DR V1 astrocyte GR-KO nuclei, ×4
RNA-seq on P28 DR V1 astrocyte GR-Con nuclei, ×4
RNA-seq on P28 DR V1 astrocyte GR-KO nuclei, ×4
RNA-seq on P14 DR V1 astrocyte GR-Con nuclei, ×3
RNA-seq on P14 NR V1 astrocyte GR-Con nuclei, ×3
RNA-seq on P14 DR V1 astrocyte GR-KO nuclei, ×3
RNA-seq on P14 NR V1 astrocyte GR-KO nuclei, ×3
+4 more
Supports a between-group comparison across 40 samples.
12 replicated groups read from 40 sample titles; they account for 40 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9195953 RNA-seq on P14 DR V1 astrocyte GR-Con nuclei, replicate 1
- GSM9195955 RNA-seq on P14 DR V1 astrocyte GR-Con nuclei, replicate 2
- GSM9195956 RNA-seq on P14 DR V1 astrocyte GR-Con nuclei, replicate 3
- GSM9195958 RNA-seq on P14 NR V1 astrocyte GR-Con nuclei, replicate 1
- GSM9195959 RNA-seq on P14 NR V1 astrocyte GR-Con nuclei, replicate 2
- GSM9195960 RNA-seq on P14 NR V1 astrocyte GR-Con nuclei, replicate 3
- GSM9195962 RNA-seq on P14 DR V1 astrocyte GR-KO nuclei, replicate 1
- GSM9195963 RNA-seq on P14 DR V1 astrocyte GR-KO nuclei, replicate 2
- GSM9195965 RNA-seq on P14 DR V1 astrocyte GR-KO nuclei, replicate 3
- GSM9195966 RNA-seq on P14 NR V1 astrocyte GR-KO nuclei, replicate 1
- GSM9195968 RNA-seq on P14 NR V1 astrocyte GR-KO nuclei, replicate 2
- GSM9195970 RNA-seq on P14 NR V1 astrocyte GR-KO nuclei, replicate 3
- GSM9195971 RNA-seq on P21 DR V1 astrocyte GR-Con nuclei, replicate 1
- GSM9195973 RNA-seq on P21 DR V1 astrocyte GR-Con nuclei, replicate 2
- GSM9195974 RNA-seq on P21 DR V1 astrocyte GR-Con nuclei, replicate 3
- GSM9195975 RNA-seq on P21 DR V1 astrocyte GR-Con nuclei, replicate 4
- GSM9195977 RNA-seq on P21 NR V1 astrocyte GR-Con nuclei, replicate 1
- GSM9195978 RNA-seq on P21 NR V1 astrocyte GR-Con nuclei, replicate 2
- GSM9195980 RNA-seq on P21 NR V1 astrocyte GR-Con nuclei, replicate 3
- GSM9195981 RNA-seq on P21 DR V1 astrocyte GR-KO nuclei, replicate 1
- GSM9195983 RNA-seq on P21 DR V1 astrocyte GR-KO nuclei, replicate 2
- GSM9195984 RNA-seq on P21 DR V1 astrocyte GR-KO nuclei, replicate 3
- GSM9195986 RNA-seq on P21 DR V1 astrocyte GR-KO nuclei, replicate 4
- GSM9195987 RNA-seq on P21 NR V1 astrocyte GR-KO nuclei, replicate 1
- GSM9195989 RNA-seq on P21 NR V1 astrocyte GR-KO nuclei, replicate 2
- GSM9195990 RNA-seq on P21 NR V1 astrocyte GR-KO nuclei, replicate 3
- GSM9195991 RNA-seq on P28 DR V1 astrocyte GR-Con nuclei, replicate 1
- GSM9195993 RNA-seq on P28 DR V1 astrocyte GR-Con nuclei, replicate 2
- GSM9195994 RNA-seq on P28 DR V1 astrocyte GR-Con nuclei, replicate 3
- GSM9195996 RNA-seq on P28 DR V1 astrocyte GR-Con nuclei, replicate 4
- GSM9195997 RNA-seq on P28 NR V1 astrocyte GR-Con nuclei, replicate 1
- GSM9195999 RNA-seq on P28 NR V1 astrocyte GR-Con nuclei, replicate 2
- GSM9196000 RNA-seq on P28 NR V1 astrocyte GR-Con nuclei, replicate 3
- GSM9196001 RNA-seq on P28 DR V1 astrocyte GR-KO nuclei, replicate 1
- GSM9196003 RNA-seq on P28 DR V1 astrocyte GR-KO nuclei, replicate 2
- GSM9196004 RNA-seq on P28 DR V1 astrocyte GR-KO nuclei, replicate 3
- GSM9196006 RNA-seq on P28 DR V1 astrocyte GR-KO nuclei, replicate 4
- GSM9196007 RNA-seq on P28 NR V1 astrocyte GR-KO nuclei, replicate 1
- GSM9196009 RNA-seq on P28 NR V1 astrocyte GR-KO nuclei, replicate 2
- GSM9196010 RNA-seq on P28 NR V1 astrocyte GR-KO nuclei, replicate 3
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