GEO series
Characterization of TLR signaling in Ticam2-/- macrophages
GSE321707
Mus musculus
Expression profiling by high throughput sequencing
30 samples
Submitted 2026/08/07
Platform GPL24247
Summary
Early Toll-like receptor (TLR) signaling termination in TRAM (Ticam2) KO macrophages selectively impairs activation of secondary response genes (SRG) and interferon-stimulated genes (ISGs), whereas primary response gene (PRG) induction remains largely intact. We performed bulk RNA-Seq on LTA- or LPS-stimulated primary WT and Ticam2-/- BMDMs after 2 h and 6 h of PAMP-treatment to analyze this phenomenom.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE321707_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 30 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1429322 and SRA study SRP679818. Searching any of these in the dataset finder brings you back here.
Study design
10 conditions, mostly in triplicate
primary TRAM (Ticam2) KO macrophages, LPS-tre… ×3
primary TRAM (Ticam2) KO macrophages, LTA-tre… ×3
primary TRAM (Ticam2) KO macrophages, ×3
primary TRAM (Ticam2) KO macrophages, LPS-tre… ×3
primary TRAM (Ticam2) KO macrophages, LTA-tre… ×3
primary WT macrophages, LPS-treated, 6 hrs, ×3
primary WT macrophages, LTA-treated, 6 hrs, ×3
primary WT macrophages, ×3
+2 more
Supports a case/control comparison: 12 samples read as cases, 3 as controls.
10 replicated groups read from 30 sample titles; they account for 30 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9549851 primary TRAM (Ticam2) KO macrophages, LPS-treated, 6 hrs, rep1
- GSM9549852 primary TRAM (Ticam2) KO macrophages, LPS-treated, 6 hrs, rep2
- GSM9549853 primary TRAM (Ticam2) KO macrophages, LPS-treated, 6 hrs, rep3
- GSM9549854 primary TRAM (Ticam2) KO macrophages, LTA-treated, 6 hrs, rep1
- GSM9549855 primary TRAM (Ticam2) KO macrophages, LTA-treated, 6 hrs, rep2
- GSM9549856 primary TRAM (Ticam2) KO macrophages, LTA-treated, 6 hrs, rep3
- GSM9549857 primary TRAM (Ticam2) KO macrophages, rep1
- GSM9549858 primary TRAM (Ticam2) KO macrophages, rep2
- GSM9549859 primary TRAM (Ticam2) KO macrophages, rep3
- GSM9549860 primary TRAM (Ticam2) KO macrophages, LPS-treated, 2 hrs, rep1
- GSM9549861 primary TRAM (Ticam2) KO macrophages, LPS-treated, 2 hrs, rep2
- GSM9549862 primary TRAM (Ticam2) KO macrophages, LPS-treated, 2 hrs, rep3
- GSM9549863 primary TRAM (Ticam2) KO macrophages, LTA-treated, 2 hrs, rep1
- GSM9549864 primary TRAM (Ticam2) KO macrophages, LTA-treated, 2 hrs, rep2
- GSM9549865 primary TRAM (Ticam2) KO macrophages, LTA-treated, 2 hrs, rep3
- GSM9549866 primary WT macrophages, LPS-treated, 6 hrs, rep1
- GSM9549867 primary WT macrophages, LPS-treated, 6 hrs, rep2
- GSM9549868 primary WT macrophages, LPS-treated, 6 hrs, rep3
- GSM9549869 primary WT macrophages, LTA-treated, 6 hrs, rep1
- GSM9549870 primary WT macrophages, LTA-treated, 6 hrs, rep2
- GSM9549871 primary WT macrophages, LTA-treated, 6 hrs, rep3
- GSM9549872 primary WT macrophages, rep1
- GSM9549873 primary WT macrophages, rep2
- GSM9549874 primary WT macrophages, rep3
- GSM9549875 primary WT macrophages, LPS-treated, 2 hrs, rep1
- GSM9549876 primary WT macrophages, LPS-treated, 2 hrs, rep2
- GSM9549877 primary WT macrophages, LPS-treated, 2 hrs, rep3
- GSM9549878 primary WT macrophages, LTA-treated, 2 hrs, rep1
- GSM9549879 primary WT macrophages, LTA-treated, 2 hrs, rep2
- GSM9549880 primary WT macrophages, LTA-treated, 2 hrs, rep3
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