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TROP2/claudin program mediates immune exclusion to impede checkpoint blockade in breast cancer

GSE334497 Mus musculus Expression profiling by high throughput sequencing 10 samples Submitted 2026/06/09 Platform GPL21103
Summary
To investigate TROP2-dependent transcriptomic changes in breast cancer, we performed RNA-seq on frozen tumor sections from 4T1 Trop2 wild-type and Trop2 knockout tumors. This dataset was generated to define transcriptional programs regulated by TROP2 in the tumor microenvironment and to determine how TROP2 contributes to immune exclusion and resistance to immune checkpoint blockade. Analysis of these tumors identified TROP2-associated enrichment of cell-cell contact and tight-junction pathways, while inflammatory, antitumor immune response, and T-cell cytotoxicity programs were relatively increased in Trop2 knockout tumors. These findings supported the conclusion that TROP2 promotes a claudin/tight-junction-mediated barrier that limits T-cell infiltration and impairs response to anti-PD-1 therapy in triple-negative breast cancer.
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Direct links to NCBI, no account and no request form: the whole study as GSE334497_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 10 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1475339 and SRA study SRP707135. Searching any of these in the dataset finder brings you back here.

Study design
5 × 4T1, KO, vs 5 × 4T1, WT,

Supports a between-group comparison across 10 samples.

2 replicated groups read from 10 sample titles; they account for 10 of them. Check it against the sample list below before relying on it.

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