GEO series
Astrocyte glucocorticoid receptor signaling restricts neuronal plasticity [CUT&RUN]
GSE306261
Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
50 samples
Submitted 2026/03/20
Platform GPL19057
Summary
Sensory experience refines neural circuits during critical periods of postnatal development. Although neuronal activity is known to orchestrate the circuit wiring that underlies this process, the environmental cues that restrain developmental plasticity as animals mature are less clear. Here, we examine the experience-dependent maturation of the mouse primary visual cortex (V1) across postnatal development using paired single-cell transcriptomic and chromatin accessibility sequencing. In addition to identifying the activity-dependent gene programs that emerge within each cortical cell type, we find that light exposure drives astrocyte maturation through cell type-specific recruitment of the glucocorticoid receptor (Nr3c1/GR) to chromatin. Astrocyte GR signaling activates an extensive gene regulatory program that is partially conserved in human brain development and promotes maturation processes that may trigger critical period closure. Collectively, these findings reveal that astrocyte GR signaling restricts neuronal plasticity. Glucocorticoid regulation of astrocyte maturation may also contribute to the effects of early-life stress across the brain, and the disruption of this process may increase susceptibility to neuropsychiatric disease.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE306261_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 50 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1310311 and SRA study SRP612457. Searching any of these in the dataset finder brings you back here.
Study design
13 conditions, mostly in triplicate
GR CUT&RUN on P14 DR V1 GFP+ astrocytes, ×3
GR CUT&RUN on P14 NR V1 GFP+ astrocytes, ×3
GR CUT&RUN on P14 NR V1 GFP+ oligodendrocytes, ×3
GR CUT&RUN on P14 NR V1 GFP+ inhibitory neuro… ×3
GR CUT&RUN on P14 NR V1 GFP+ excitatory neuro… ×3
NFIA CUT&RUN on P14 NR V1 GFP+ astrocytes, ×3
NFIA CUT&RUN on P14 NR V1 GFP+ oligodendrocyt… ×3
NFIA CUT&RUN on P14 NR V1 GFP+ inhibitory neu… ×3
+10 more
Supports a between-group comparison across 35 samples.
13 replicated groups read from the first 40 of 50 sample titles; they account for 35 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9195872 GR CUT&RUN on P14 DR V1 GFP+ astrocytes, replicate 1
- GSM9195873 GR CUT&RUN on P14 DR V1 GFP+ astrocytes, replicate 2
- GSM9195875 GR CUT&RUN on P14 DR V1 GFP+ astrocytes, replicate 3
- GSM9195876 GR CUT&RUN on P14 NR V1 GFP+ astrocytes, replicate 1
- GSM9195877 GR CUT&RUN on P14 NR V1 GFP+ astrocytes, replicate 2
- GSM9195879 GR CUT&RUN on P14 NR V1 GFP+ astrocytes, replicate 3
- GSM9195880 GR CUT&RUN on P14 DR V1 GFP- cells, replicate 1
- GSM9195882 GR CUT&RUN on P14 DR V1 GFP- cells, replicate 2
- GSM9195883 GR CUT&RUN on P14 NR V1 GFP- cells, replicate 1
- GSM9195885 GR CUT&RUN on P14 NR V1 GFP- cells, replicate 2
- GSM9195886 IgG CUT&RUN on P14 DR V1 GFP+ astrocytes, replicate 1
- GSM9195887 IgG CUT&RUN on P14 NR V1 GFP+ astrocytes, replicate 1
- GSM9195889 GR CUT&RUN on P14 NR V1 GR-Con astrocytes, replicate 1
- GSM9195890 GR CUT&RUN on P14 NR V1 GR-Con astrocytes, replicate 2
- GSM9195892 GR CUT&RUN on P14 NR V1 GR-KO astrocytes, replicate 1
- GSM9195893 GR CUT&RUN on P14 NR V1 GR-KO astrocytes, replicate 2
- GSM9195894 GR CUT&RUN on P14 NR V1 GFP+ oligodendrocytes, replicate 1
- GSM9195896 GR CUT&RUN on P14 NR V1 GFP+ oligodendrocytes, replicate 2
- GSM9195897 GR CUT&RUN on P14 NR V1 GFP+ oligodendrocytes, replicate 3
- GSM9195899 GR CUT&RUN on P14 NR V1 GFP+ inhibitory neurons, replicate 1
- GSM9195900 GR CUT&RUN on P14 NR V1 GFP+ inhibitory neurons, replicate 2
- GSM9195902 GR CUT&RUN on P14 NR V1 GFP+ inhibitory neurons, replicate 3
- GSM9195903 GR CUT&RUN on P14 NR V1 GFP+ excitatory neurons, replicate 1
- GSM9195905 GR CUT&RUN on P14 NR V1 GFP+ excitatory neurons, replicate 2
- GSM9195906 GR CUT&RUN on P14 NR V1 GFP+ excitatory neurons, replicate 3
- GSM9195907 NFIA CUT&RUN on P14 NR V1 GFP+ astrocytes, replicate 1
- GSM9195909 NFIA CUT&RUN on P14 NR V1 GFP+ astrocytes, replicate 2
- GSM9195910 NFIA CUT&RUN on P14 NR V1 GFP+ astrocytes, replicate 3
- GSM9195912 NFIA CUT&RUN on P14 NR V1 GFP+ oligodendrocytes, replicate 1
- GSM9195913 NFIA CUT&RUN on P14 NR V1 GFP+ oligodendrocytes, replicate 2
- GSM9195914 NFIA CUT&RUN on P14 NR V1 GFP+ oligodendrocytes, replicate 3
- GSM9195916 NFIA CUT&RUN on P14 NR V1 GFP+ inhibitory neurons, replicate 1
- GSM9195917 NFIA CUT&RUN on P14 NR V1 GFP+ inhibitory neurons, replicate 2
- GSM9195919 NFIA CUT&RUN on P14 NR V1 GFP+ inhibitory neurons, replicate 3
- GSM9195920 NFIA CUT&RUN on P14 NR V1 GFP+ excitatory neurons, replicate 1
- GSM9195921 NFIA CUT&RUN on P14 NR V1 GFP+ excitatory neurons, replicate 2
- GSM9195923 NFIA CUT&RUN on P14 NR V1 GFP+ excitatory neurons, replicate 3
- GSM9195924 IgG CUT&RUN on P14 NR V1 GFP+ oligodendrocytes, replicate 1
- GSM9195926 IgG CUT&RUN on P14 NR V1 GFP+ inhibitory neurons, replicate 1
- GSM9195927 IgG CUT&RUN on P14 NR V1 GFP+ excitatory neurons, replicate 1
+ 10 more — browse all 50 samples with per-sample file links →
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