GEO series
HP1B and H3K9me3 Regulate Olfactory Receptor Choice and 2 Transcriptional Identity [ChIP-seq]
GSE324864
Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
28 samples
Submitted 2026/04/22
Platform GPL21626
Summary
Diverse epigenetic regulatory mechanisms ensure and regulate cellular diversity. Among others, the histone 3 lysine 9 me3 (H3K9me3) post translational modification participates in silencing lineage-inappropriate genes. H3K9me3 restricts access of transcription factors and other regulatory proteins to genes that control cell fate. Mouse olfactory sensory neurons (OSN) select to express one olfactory receptor (OR) gene out of 2,600 possibilities. This monoallelic and stochastic OR choice happens as OSNs differentiate and undergo dramatic changes in nuclear architecture. OR genes from different chromosomes converge into specialized nuclear bodies and chromatin compartments, as H3K9me3 and chromatin binding proteins including heterochromatin protein 1 (HP1) are incorporated. In this work, we have uncovered an unexpected role for HP1b in OR choice and neuronal identity that cannot be rescued by HP1a in vivo. With the use of a conditional knock-in mouse model that replaces HP1b for HP1a, we observe changes in H3K9me3 levels and DNA accessibility over OR gene clusters. These changes alter the expression paTerns that partition the mouse olfactory epithelium into five OR expression zones, which results in a reduced OR repertoire leading to a loss of olfactory sensory neuron diversity. We propose that HP1b modulates the competition of OR-promoters for enhancers to promote receptor diversity, by establishing repression gradients in a zonal fashion.
Published in
Escamilla-Del-Arenal M, Duffié R, Shayya H et al.
· International journal of molecular sciences 2026
· PMID 41977146
· doi:10.3390/ijms27072958
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE324864_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 28 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1438019 and SRA study SRP683980. Searching any of these in the dataset finder brings you back here.
Study design
9 conditions, mostly in triplicate
H3K9me3 ChIP, Tissue zone 1, Swap mouse ×3
H3K9me3 ChIP, Tissue zone 1, control mouse ×3
H3K9me3 ChIP, Tissue zone 5, Swap mouse ×3
H3K9me3 ChIP, Tissue zone 5, control mouse ×3
H3K9me3 ChIP, OMP-GFP- Tissue zone 1, control… ×3
H3K9me3 ChIP, OMP-GFP- Tissue zone 5, control… ×3
H3K9me3 ChIP, OMP-GFP- Tissue zone 1, Swap mo… ×3
H3K9me3 ChIP, OMP-GFP- Tissue zone 5, Swap mo… ×3
+3 more
Supports a between-group comparison across 26 samples.
9 replicated groups read from 28 sample titles; they account for 26 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9587919 H3K9me3 ChIP, Tissue zone 1, Swap mouse Replicate 1
- GSM9587920 H3K9me3 ChIP, Tissue zone 1, Swap mouse Replicate 2
- GSM9587921 H3K9me3 ChIP, Tissue zone 1, Swap mouse Replicate 3
- GSM9587922 H3K9me3 ChIP, Tissue zone 1, control mouse Replicate 1
- GSM9587923 H3K9me3 ChIP, Tissue zone 1, control mouse Replicate 2
- GSM9587924 H3K9me3 ChIP, Tissue zone 1, control mouse Replicate 3
- GSM9587925 H3K9me3 ChIP, Tissue zone 5, Swap mouse Replicate 1
- GSM9587926 H3K9me3 ChIP, Tissue zone 5, Swap mouse Replicate 2
- GSM9587927 H3K9me3 ChIP, Tissue zone 5, Swap mouse Replicate 3
- GSM9587928 H3K9me3 ChIP, Tissue zone 5, control mouse Replicate 1
- GSM9587929 H3K9me3 ChIP, Tissue zone 5, control mouse Replicate 2
- GSM9587930 H3K9me3 ChIP, Tissue zone 5, control mouse Replicate 3
- GSM9587931 Input control_1
- GSM9587932 Input control_2
- GSM9587933 H3K9me3 ChIP, OMP-GFP- Tissue zone 1, control mouse Replicate 1
- GSM9587934 H3K9me3 ChIP, OMP-GFP- Tissue zone 1, control mouse Replicate 2
- GSM9587935 H3K9me3 ChIP, OMP-GFP- Tissue zone 1, control mouse Replicate 3
- GSM9587936 H3K9me3 ChIP, OMP-GFP- Tissue zone 5, control mouse Replicate 1
- GSM9587937 H3K9me3 ChIP, OMP-GFP- Tissue zone 5, control mouse Replicate 2
- GSM9587938 H3K9me3 ChIP, OMP-GFP- Tissue zone 5, control mouse Replicate 3
- GSM9587939 H3K9me3 ChIP, OMP-GFP- Tissue zone 1, Swap mouse Replicate 1
- GSM9587940 H3K9me3 ChIP, OMP-GFP- Tissue zone 1, Swap mouse Replicate 2
- GSM9587941 H3K9me3 ChIP, OMP-GFP- Tissue zone 1, Swap mouse Replicate 3
- GSM9587942 H3K9me3 ChIP, OMP-GFP- Tissue zone 5, Swap mouse Replicate 1
- GSM9587943 H3K9me3 ChIP, OMP-GFP- Tissue zone 5, Swap mouse Replicate 2
- GSM9587944 H3K9me3 ChIP, OMP-GFP- Tissue zone 5, Swap mouse Replicate 3
- GSM9587945 H3K9me3 ChIP, OMP-GFP- InPut Control for Cut and Run
- GSM9587946 H3K9me3 ChIP, OMP-GFP- Peaks Reference Control for Cut and Run
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