GEO series
Mechanism of maintenance and establishment of repression by the Mtg16 tumor suppressor [CUT&RUN, ChIP-Seq]
GSE303384
Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
92 samples
Submitted 2026/07/01
Platform GPL34328
Summary
The MTG family of transcriptional corepressors are frequent targets of chromosomal translocations and deletions in cancer, yet the mechanisms underlying tumor suppression are unclear. We employed a chemical-genetic approach to investigate the molecular mechanism of Mtg16-mediated repression. Analysis of nascent transcription revealed that Mtg16 is required to repress hundreds of genes and enhancers, including the oncogenes Myb and Gfi1b. Because Mtg16 tightly regulated its own transcription, this model yielded a rapid “on/off/on” switch for Mtg16, providing a unique opportunity to study maintenance and establishment of repression within the same system. Mtg16 directly opposed histone acetylation at regulated loci, but analysis of H3K27ac revealed that changes in this mark correlated better with Mtg16 itself than with transcriptional activity. Importantly, both the relationship between Mtg16 and Class I HDAC activity and Mtg16-mediated re-establishment of repression were dependent target transcription. We propose that Mtg16 acts as a transcription-sensitive damper at highly accessible loci.
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE303384_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 92 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1294979 and SRA study SRP602782. Searching any of these in the dataset finder brings you back here.
Study design
40 conditions, each sampled once — no replicated groups
Read from the first 40 of 92 sample titles: 40 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9124871 MEL_Mtg16-FKBP_H3K27ac-ChIP_DMSO_A
- GSM9124872 MEL_Mtg16-FKBP_H3K27ac-ChIP_DMSO_B
- GSM9124873 MEL_Mtg16-FKBP_H3K27ac-ChIP_DMSO_C
- GSM9124874 MEL_Mtg16-FKBP_H3K27ac-ChIP_15m_A
- GSM9124875 MEL_Mtg16-FKBP_H3K27ac-ChIP_15m_B
- GSM9124876 MEL_Mtg16-FKBP_H3K27ac-ChIP_15m_C
- GSM9124877 MEL_Mtg16-FKBP_H3K27ac-ChIP_30m_A
- GSM9124878 MEL_Mtg16-FKBP_H3K27ac-ChIP_30m_B
- GSM9124879 MEL_Mtg16-FKBP_H3K27ac-ChIP_30m_C
- GSM9124880 MEL_Mtg16-FKBP_H3K27ac-ChIP_1h_A
- GSM9124881 MEL_Mtg16-FKBP_H3K27ac-ChIP_1h_B
- GSM9124882 MEL_Mtg16-FKBP_H3K27ac-ChIP_1h_C
- GSM9124883 MEL_Mtg16-FKBP_H3K27ac-ChIP_2h_A
- GSM9124884 MEL_Mtg16-FKBP_H3K27ac-ChIP_2h_B
- GSM9124885 MEL_Mtg16-FKBP_H3K27ac-ChIP_2h_C
- GSM9124886 MEL_Mtg16-FKBP_H3K27ac-ChIP_30mWO_A
- GSM9124887 MEL_Mtg16-FKBP_H3K27ac-ChIP_30mWO_B
- GSM9124888 MEL_Mtg16-FKBP_H3K27ac-ChIP_30mWO_C
- GSM9124889 MEL_Mtg16-FKBP_H3K27ac-ChIP_1hWO_A
- GSM9124890 MEL_Mtg16-FKBP_H3K27ac-ChIP_1hWO_B
- GSM9124891 MEL_Mtg16-FKBP_H3K27ac-ChIP_1hWO_C
- GSM9124892 MEL_Mtg16-FKBP_H3K27ac-ChIP_2hWO_A
- GSM9124893 MEL_Mtg16-FKBP_H3K27ac-ChIP_2hWO_B
- GSM9124894 MEL_Mtg16-FKBP_H3K27ac-ChIP_2hWO_C
- GSM9124895 MEL Mtg16 H3K4me1 DMSO A
- GSM9124896 MEL Mtg16 H3K4me1 DMSO B
- GSM9124897 MEL Mtg16 H3K4me1 DMSO C
- GSM9124898 MEL Mtg16 H3K4me1 dTAGV1 15m A
- GSM9124899 MEL Mtg16 H3K4me1 dTAGV1 15m B
- GSM9124900 MEL Mtg16 H3K4me1 dTAGV1 15m C
- GSM9124901 MEL Mtg16 H3K4me1 dTAGV1 30m A
- GSM9124902 MEL Mtg16H3K4me1 dTAGV1 30m B
- GSM9124903 MEL Mtg16 H3K4me1 dTAGV1 30m C
- GSM9124904 MEL Mtg16 H3K4me1 dTAGV1 1h A
- GSM9124905 MEL Mtg16 H3K4me1 dTAGV1 1h B
- GSM9124906 MEL Mtg16 H3K4me1 dTAGV1 1h C
- GSM9124907 MEL Mtg16 H3K4me1 dTAGV1 2h A
- GSM9124908 MEL Mtg16 H3K4me1 dTAGV1 2h B
- GSM9124909 MEL Mtg16 H3K4me1 dTAGV1 2h C
- GSM9124910 MEL Mtg16 H3K4me1 30mWO A
+ 52 more — browse all 92 samples with per-sample file links →
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