GEO series
Cell autonomous regional differences in oligodendrocyte precursor cell development and response to oncohistone H3.3 K27M [ChIP-Seq]
GSE310474
Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
40 samples
Submitted 2026/05/22
Platform GPL24247
Summary
Diffuse midline glioma (DMG) is an incurable tumor arising in brainstem and midline structures. Most DMGs harbor missense histone H3 mutations replacing lysine 27 with methionine (H3 K27M). The mechanisms underlying regional selectivity for H3 K27M mutations, which are found almost exclusively in DMG, are not well understood. Here, we use a conditional knock-in mouse model to probe the postnatal brain region-specific oncogenic effects of H3.3 K27M in embryonic GFAP+ neural stem cells. We show that H3.3 K27M reduces oligodendrocyte differentiation in a region-specific manner and increases postnatal proliferation of distinct progenitor states by brain region in vivo, selectively expanding the oligodendrocyte-lineage in the ventral pons. Cellular morphology, marker expression, and transcriptomic analyses of primary region-specific mouse OPC cultures demonstrated cell-intrinsic differences in wild-type brainstem and forebrain OPC differentiation dynamics. H3.3 K27M drives cell-autonomous increase in brainstem-derived OPC proliferation and disrupts intrinsic region-specific transcriptional programs, delaying differentiation in forebrain-derived OPCs and restraining full maturation of brainstem-derived OPCs. H3.3 K27M-mediated changes include brainstem-selective upregulation of genes in Bmp, Wnt, and Notch pathways that are relevant in DMG pathogenesis. Our findings reveal that H3.3 K27M differentially disrupts cell-autonomous regional oligodendrocyte development, which may provide an opportunistic window for brainstem-selective gliomagenesis.
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE310474_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 40 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1366412 and SRA study SRP646867. Searching any of these in the dataset finder brings you back here.
Study design
40 conditions, each sampled once — no replicated groups
Read from 40 sample titles: 40 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9301935 K27M brainstem OPC culture 59, no differentiation, H3K27me3 ChIP
- GSM9301936 K27M brainstem OPC culture 59, no differentiation, Input for H3K27me3 ChIP
- GSM9301937 K27M brainstem OPC culture 59, no differentiation, H3K4me3 ChIP
- GSM9301938 K27M brainstem OPC culture 59, no differentiation, Input for H3K4me3 ChIP
- GSM9301939 Control brainstem OPC culture 59, no differentiation, H3K27me3 ChIP
- GSM9301940 Control brainstem OPC culture 59, no differentiation, Input for H3K27me3 ChIP
- GSM9301941 Control brainstem OPC culture 59, no differentiation, H3K4me3 ChIP
- GSM9301942 Control brainstem OPC culture 59, no differentiation, Input for H3K4me3 ChIP
- GSM9301943 K27M brainstem OPC culture 61, no differentiation, H3K27me3 ChIP
- GSM9301944 K27M brainstem OPC culture 61, no differentiation, Input for H3K27me3 ChIP
- GSM9301945 K27M brainstem OPC culture 61, no differentiation, H3K4me3 ChIP
- GSM9301946 K27M brainstem OPC culture 61, no differentiation, Input for H3K4me3 ChIP
- GSM9301947 Control brainstem OPC culture 61, no differentiation, H3K27me3 ChIP
- GSM9301948 Control brainstem OPC culture 61, no differentiation, Input for H3K27me3 ChIP
- GSM9301949 Control brainstem OPC culture 61, no differentiation, H3K4me3 ChIP
- GSM9301950 Control brainstem OPC culture 61, no differentiation, Input for H3K4me3 ChIP
- GSM9301951 K27M cortex OPC culture 59, no differentiation, H3K27me3 ChIP
- GSM9301952 K27M cortex OPC culture 59, no differentiation, Input for H3K27me3 ChIP
- GSM9301953 K27M cortex OPC culture 59, no differentiation, H3K4me3 ChIP
- GSM9301954 K27M cortex OPC culture 59, no differentiation, Input for H3K4me3 ChIP
- GSM9301955 Control cortex OPC culture 59, no differentiation, H3K27me3 ChIP
- GSM9301956 Control cortex OPC culture 59, no differentiation, Input for H3K27me3 ChIP
- GSM9301957 Control cortex OPC culture 59, no differentiation, H3K4me3 ChIP
- GSM9301958 Control cortex OPC culture 59, no differentiation, Input for H3K4me3 ChIP
- GSM9301959 K27M cortex OPC culture 60, no differentiation, H3K27me3 ChIP
- GSM9301960 K27M cortex OPC culture 60, no differentiation, Input for H3K27me3 ChIP
- GSM9301961 K27M cortex OPC culture 60, no differentiation, H3K4me3 ChIP
- GSM9301962 K27M cortex OPC culture 60, no differentiation, Input for H3K4me3 ChIP
- GSM9301963 Control cortex OPC culture 60, no differentiation, H3K27me3 ChIP
- GSM9301964 Control cortex OPC culture 60, no differentiation, Input for H3K27me3 ChIP
- GSM9301965 Control cortex OPC culture 60, no differentiation, H3K4me3 ChIP
- GSM9301966 Control cortex OPC culture 60, no differentiation, Input for H3K4me3 ChIP
- GSM9301967 K27M cortex OPC culture 61, no differentiation, H3K27me3 ChIP
- GSM9301968 K27M cortex OPC culture 61, no differentiation, Input for H3K27me3 ChIP
- GSM9301969 K27M cortex OPC culture 61, no differentiation, H3K4me3 ChIP
- GSM9301970 K27M cortex OPC culture 61, no differentiation, Input for H3K4me3 ChIP
- GSM9301971 Control cortex OPC culture 61, no differentiation, H3K27me3 ChIP
- GSM9301972 Control cortex OPC culture 61, no differentiation, Input for H3K27me3 ChIP
- GSM9301973 Control cortex OPC culture 61, no differentiation, H3K4me3 ChIP
- GSM9301974 Control cortex OPC culture 61, no differentiation, Input for H3K4me3 ChIP
Similar datasets
- GSE295340 Polycomb Chromatin Topology Enables Long-Range Enhancer Recruitment during Craniofacial Development [ChIP-seq] 42 samples
- GSE266022 Nrf2-mediated chromatin activation of hyperosmotic response genes attenuates heat-induced renal injury [ChIP-seq] 24 samples
- GSE261221 Scaffolding element rewires genome architecture during differentiation at the Zfp608 locus (ChIP-Seq) 189 samples
- GSE303384 Mechanism of maintenance and establishment of repression by the Mtg16 tumor suppressor [CUT&RUN, ChIP-Seq] 92 samples
- GSE272524 Chromatin-dependent motif syntax defines differentiation trajectories [ChIP-seq] 76 samples
- GSE294085 SIRT6 Overexpression Counteracts Chromatin Aging [ChIP-Seq] 74 samples
- GSE297574 H3K9 di-methylation dynamics underlies mouse minor zygotic genome activation [spike-in ChIP-seq of mESC] 64 samples
- GSE289108 Neurons undergo IFNγ-driven persistent epigenetic shifts and synaptic remodeling in the inflamed brain [ChIP-Seq] 57 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.