GEO series
The RNA binding protein LIN28A mediates chromatin dynamics during neuronal differentiation
GSE306869
Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
24 samples
Submitted 2026/05/01
Platform GPL24247
Summary
The transition of embryonic stem cells (ESCs) from pluripotency to lineage commitment involves complex regulatory mechanisms, including chromatin dynamics and transcriptional/post- transcriptional processes. These mechanisms often interact within intricate networks that require thorough investigation. In this study, we highlight the critical role of the mouse RNA-binding protein LIN28A in neuronal differentiation. LIN28A mediates RNA-dependent interactions with thePolycomb repressive complex 2 (PRC2), leading to the eviction of PRC2 from chromatin andactivation of a neuronal lineage-specific transcriptional program. Proteomic analyses revealed thatthe LIN28A interactome undergoes substantial remodeling during differentiation, corresponding to changes in LIN28A localization. In undifferentiated ESCs, LIN28A primarily resides in the nucleus,interacting with PRC2 components in an RNA-dependent manner, assisting in chromatin dynamics. The absence of LIN28A results in persistent PRC2 association with chromatin, impairing theexpression of genes critical for neuronal differentiation. Chromatin immunoprecipitationsequencing further confirmed that loss of LIN28A results in preferential PRC2 occupancy at thepromoters of differentiation-associated genes. These findings reveal a novel role of LIN28A inepigenetic remodeling, crucial for proper neuronal differentiation of ESCs.
Published in
Piscitelli S, Cascone E, D'Ambrosio C et al.
· Cell death and differentiation 2026
· PMID 42086780
· doi:10.1038/s41418-026-01753-2
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE306869_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 24 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1312830 and SRA study SRP614748. Searching any of these in the dataset finder brings you back here.
Study design
24 conditions, each sampled once — no replicated groups
Read from 24 sample titles: 24 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9211078 H3K27ME3 KO ESC 1 (Chip)
- GSM9211079 H3K27ME3 KO ESC 1 (IP)
- GSM9211080 H3K27ME3 KO ESC 2 (Chip)
- GSM9211081 H3K27ME3 KO ESC 2 (IP)
- GSM9211082 H3K27ME3 KO ESC 3 (Chip)
- GSM9211083 H3K27ME3 KO ESC 3 (IP)
- GSM9211084 H3K27ME3 WT ESC 1 (Chip)
- GSM9211085 H3K27ME3 WT ESC 1 (Input)
- GSM9211086 H3K27ME3 WT ESC2 (Chip)
- GSM9211087 H3K27ME3 WT ESC 2 (Input)
- GSM9211088 H3K27ME3 WT ESC 3 (Chip)
- GSM9211089 H3K27ME3 WT ESC 3 (Input)
- GSM9211090 H3K27ME3 KO sFEB 1(Chip)
- GSM9211091 H3K27ME3 KO sFEB 1 (Input)
- GSM9211092 H3K27ME3 KO sFEB 2 (Chip)
- GSM9211093 H3K27ME3 KO sFEB 2 (Input)
- GSM9211094 H3K27ME3 KO sFEB 3 (Chip)
- GSM9211095 H3K27ME3 KO sFEB 3 (Input)
- GSM9211096 H3K27ME3 WT sFEB 1 (Chip)
- GSM9211097 H3K27ME3 WT sFEB 1 (Input)
- GSM9211098 H3K27ME3 WT sFEB 2 (Chip)
- GSM9211099 H3K27ME3 WT sFEB 2 (Input)
- GSM9211100 H3K27ME3 WT sFEB 3 (Chip)
- GSM9211101 H3K27ME3 WT sFEB 3 (Input)
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