GEO series
H4K16ac contributes to chromatin compartment reorganization during mitotic and meiotic transitions [CUTnTAG]
GSE304211
Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
128 samples
Submitted 2026/05/27
Platform GPL24247
Summary
Chromatin compartments reorganize dramatically during cell cycle transitions, shifting from a compartmentalized interphase to a condensed mitotic configuration in which large-scale compartment signals are attenuated. While histone modifications are linked to compartment identity, their contribution to compartment organization remains unclear. Using meiotic (mouse spermatogenesis) and mitotic (mouse neuronal progenitor cell, mNPC) models, we mapped histone landscapes alongside compartment dynamics. We found that histone H4 lysine 16 acetylation (H4K16ac) strongly tracked compartment reorganization, remaining enriched in A compartments during compartmentalization but redistributing upon chromatin compaction. In spermatogenesis, H4K16ac loss on the X chromosome coincided with condensation and H3K9me3 spreading. Machine learning highlighted H4K16ac as a stronger predictor of compartment transitions than H3K27ac. Acute degradation of MOF, the principal H4K16ac acetyltransferase, disrupted both the spatial distribution and global abundance of H4K16ac and progressively impaired compartmentalization in mNPCs. These results position H4K16ac as a histone modification selectively coupled to chromatin compartment transitions.
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE304211_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 128 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1299391 and SRA study SRP605474. Searching any of these in the dataset finder brings you back here.
Study design
19 conditions, mostly in duplicate
SPD, WT, H4K16ac, ×3
mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 4h), H4K… ×2
SPG, WT, H3K4me3, ×2
mNPC , MOF-dTAG, dTAG13 (500 nM, 0h), RNAP, ×2
SPD, WT, H3K9me3, ×2
mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 0h), H4K… ×2
mNPC G2_M, MOF-dTAG, dTAG13 (500 nM, 0h), H3K… ×2
mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 0h), H3K… ×2
+12 more
Supports a between-group comparison across 39 samples.
19 replicated groups read from the first 40 of 128 sample titles; they account for 39 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9145098 mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 4h), H4K16ac, rep2
- GSM9145099 mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 4h), H4K16ac, rep1
- GSM9145100 SPG, WT, H3K4me3, rep2
- GSM9145101 SPG, WT, H3K4me3, rep1
- GSM9145102 mNPC , MOF-dTAG, dTAG13 (500 nM, 0h), RNAP, rep1
- GSM9145103 mNPC , MOF-dTAG, dTAG13 (500 nM, 0h), RNAP, rep2
- GSM9145104 SPD, WT, H3K9me3, rep3
- GSM9145105 mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 0h), H4K16ac, rep1
- GSM9145106 mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 0h), H4K16ac, rep2
- GSM9145107 mNPC G2_M, MOF-dTAG, dTAG13 (500 nM, 0h), H3K27me3, rep1
- GSM9145108 mNPC G2_M, MOF-dTAG, dTAG13 (500 nM, 0h), H3K27me3, rep2
- GSM9145109 mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 0h), H3K27ac, rep2
- GSM9145110 mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 0h), H3K27ac, rep1
- GSM9145111 SPD, WT, H4K16ac, rep1
- GSM9145112 SPD, WT, H4K16ac, rep2
- GSM9145113 mNPC LS, MOF-dTAG, dTAG13 (500 nM, 0h), H3K27ac, rep2
- GSM9145114 mNPC LS, MOF-dTAG, dTAG13 (500 nM, 0h), H3K27ac, rep1
- GSM9145115 MI, WT, RNAPIIser2, rep2
- GSM9145116 MI, WT, RNAPIIser2, rep1
- GSM9145117 mNPC LS, MOF-dTAG, dTAG13 (500 nM, 0h), H3K9me3, rep2
- GSM9145118 mNPC LS, MOF-dTAG, dTAG13 (500 nM, 0h), H3K9me3, rep1
- GSM9145119 SPG, WT, H3K9me3, rep2
- GSM9145120 SPG, WT, H3K9me3, rep1
- GSM9145121 mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 4h), TSA (330 nM, 4h), H4K16ac, rep1
- GSM9145122 mNPC G0_1, MOF-dTAG, dTAG13 (500 nM, 4h), TSA (330 nM, 4h), H4K16ac, rep2
- GSM9145123 mNPC LS, MOF-dTAG, dTAG13 (500 nM, 0h), H4K16ac, rep1
- GSM9145124 mNPC LS, MOF-dTAG, dTAG13 (500 nM, 0h), H4K16ac, rep2
- GSM9145125 SPD, WT, H3K9me3, rep1
- GSM9145126 mNPC ES, MOF-dTAG, dTAG13 (500 nM, 0h), H3K9me3, rep1
- GSM9145127 mNPC ES, MOF-dTAG, dTAG13 (500 nM, 0h), H3K9me3, rep2
- GSM9145128 MI, WT, H4K16ac, rep1
- GSM9145129 MI, WT, H4K16ac, rep2
- GSM9145130 mNPC G2_M, MOF-dTAG, dTAG13 (500 nM, 4h), TSA (330 nM, 4h), H4K16ac, rep1
- GSM9145131 mNPC G2_M, MOF-dTAG, dTAG13 (500 nM, 4h), TSA (330 nM, 4h), H4K16ac, rep2
- GSM9145132 mNPC ES, MOF-dTAG, dTAG13 (500 nM, 0h), H4K16ac, rep2
- GSM9145133 mNPC ES, MOF-dTAG, dTAG13 (500 nM, 0h), H4K16ac, rep1
- GSM9145134 MII, WT, H4K16ac, rep2
- GSM9145135 MII, WT, H4K16ac, rep1
- GSM9145136 SPD, WT, H4K16ac, rep3
- GSM9145137 mNPC ES, MOF-dTAG, dTAG13 (500 nM, 0h), H3K27ac, rep1
+ 88 more — browse all 128 samples with per-sample file links →
Similar datasets
- GSE295340 Polycomb Chromatin Topology Enables Long-Range Enhancer Recruitment during Craniofacial Development [ChIP-seq] 42 samples
- GSE306869 The RNA binding protein LIN28A mediates chromatin dynamics during neuronal differentiation 24 samples
- GSE292285 Depletion of lamin-associated polypeptide 2 alpha leads to chromatin reorganization and redistribution of A-type lamins to open genomic regions [ChIP-seq] 22 samples
- GSE261221 Scaffolding element rewires genome architecture during differentiation at the Zfp608 locus (ChIP-Seq) 189 samples
- GSE272524 Chromatin-dependent motif syntax defines differentiation trajectories [ChIP-seq] 76 samples
- GSE294085 SIRT6 Overexpression Counteracts Chromatin Aging [ChIP-Seq] 74 samples
- GSE293129 Enhancer rewiring orchestrates inflammation and loss of cell identity during muscle stem cell aging [CUT&RUN] 36 samples
- GSE298001 Stage-specific Epigenetic Priming Amplifies Gene Activation During Lineage Commitment [ATAC-Seq] 36 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.