GEO series

Nup153 regulates neuronal responsiveness through HDAC1-mediated epigenetic modulation [ChIP-seq]

GSE275917 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 17 samples Submitted 2026/07/31 Platform GPL24247

Summary

Neural activity-dependent gene regulation is central to the development of neural networks and neuronal plasticity. Induction of activity-dependent gene programs is equally important as repression of these programs, and both need to be balanced carefully. However, little is known about how repressive mechanisms modulate neuronal responsiveness across the genome before stimulation. Here, we identify nucleoporin-dependent regulation of neuronal responsiveness in which Nup153 represses neuronal genes including activity-regulated genes (ARGs) in the basal state. By characterizing the genome-wide landscape of chromatin accessibility, histone modifications and Nup153 chromatin binding, we show that Nup153 directly influences their target genes and associated chromatin states through both basal activity-dependent and -independent mechanisms. Mechanistically, Nup153 recruits HDAC1 to regulate histone acetylation and repress target chromatin regions. We propose that Nup153 acts as a structural platform to recruit epigenetic repressor to maintain activity-dependent programs in the poised state, thus maintaining neuronal responsiveness as a gatekeeper.

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Direct links to NCBI, no account and no request form: the whole study as GSE275917_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 17 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1152568 and SRA study SRP528678. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

7 conditions, mostly in duplicate

CHIP_Basal_HDAC1 ×2 CHIP_Basal_Input ×2 CHIP_H3K27ac_Basal_shControl ×2 CHIP_H3K27ac_Basal_shNup153_2 ×2 CHIP_Input_Basal_shNup153_2 ×2 CHIP_H3K27ac_TTX_AP5_shControl ×2 CHIP_H3K27ac_TTX_AP5_shNup153_2 ×2 CHIP_Input_Basal_shControl ×1 +2 more

Supports a between-group comparison across 14 samples.

7 replicated groups read from 17 sample titles; they account for 14 of them. Check it against the sample list below before relying on it.

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