← BioTransfer GEO Dataset Finder
GEO series

The C3-C3aR axis modulates trained immunity in alveolar macrophages

GSE281001 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/08/07 Platform GPL24247
Summary
Complement protein C3 is crucial for immune responses in mucosal sites like the respiratory system, where it aids in microbe elimination and enhances inflammation. While trained immunity – enhanced secondary responses of innate immune cells after prior exposure – is well-studied, the role of the complement system in trained immune responses remains unclear. We investigated the role of C3 in trained immunity and found that in vivo, trained wild-type mice showed significantly elevated pro-inflammatory cytokines and increased C3a levels upon a second stimulus, whereas C3-deficient mice exhibited a blunted cytokine response and heightened evidence of lung injury. Ex vivo, C3-deficient alveolar macrophages (AMs) displayed reduced chemokine and cytokine output after training, which was restored by exogenous C3 but not by C3a. Inhibiting C3aR, both pharmacologically and with a genetic C3aR knockout, prevented this restoration, indicating the necessity of C3aR engagement. Mechanistically, trained WT AMs demonstrated enhanced glycolytic activity compared to C3-deficient AMs – a defect corrected by exogenous C3 in a C3aR-dependent manner. These findings reveal that C3 modulates trained immunity in AMs through C3aR signaling, affecting cytokine production and metabolic reprogramming, and highlight a novel role for C3 in trained immunity.
This dataset
Open GSE281001 in finder → Find more mouse RNA-seq datasets →
Download

Direct links to NCBI, no account and no request form: the whole study as GSE281001_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1181815 and SRA study SRP543091. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
4 × WT_trained vs 4 × C3KO_trained

Supports a between-group comparison across 8 samples.

2 replicated groups read from 8 sample titles; they account for 8 of them. Check it against the sample list below before relying on it.

Samples in this study
Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.