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Immunogen-Sponge Hydrogel Reverses TIM-3–Mediated Dendritic-Cell Exhaustion in Alveolar Echinococcosis

GSE334769 Mus musculus Expression profiling by high throughput sequencing 12 samples Submitted 2026/08/08 Platform GPL28330
Summary
Alveolar echinococcosis (AE) induces profound dendritic-cell dysfunction characterized by elevated TIM-3 expression and impaired antigen presentation. To overcome parasite-induced immunosuppression, we developed a SHIFT-engineered Immunogen-Sponge hydrogel vaccine incorporating protoscolex-derived antigens (PSAs). Bone marrow-derived dendritic cells (BMDCs) were treated with saline, PSA, F127@PSA, or SHIFT-F127@PSA and subjected to transcriptome sequencing. Comparative transcriptomic analyses were performed to investigate dendritic-cell activation, antigen presentation, immune signaling pathways, and TIM-3-associated regulatory mechanisms.
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Direct links to NCBI, no account and no request form: the whole study as GSE334769_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 12 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1476314 and SRA study SRP707874. Searching any of these in the dataset finder brings you back here.

Study design
3 × BMDC_Saline vs 3 × BMDC_PSA vs 3 × BMDC_F127 vs 3 × BMDC_SHIFT

Supports a between-group comparison across 12 samples.

4 replicated groups read from 12 sample titles; they account for 12 of them. Check it against the sample list below before relying on it.

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