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RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues, cancer cells, and macrophages [RNA-Seq and Ribo-Seq]

GSE274188 Homo sapiens Other; Expression profiling by high throughput sequencing 12 samples Submitted 2024/09/10 Platform GPL24676
Summary
Ribosomes are emerging as direct regulators of gene expression, with ribosome-associated proteins (RAPs) allowing ribosomes to modulate translation. Nevertheless, a lack of technologies to enrich RAPs across sample types has prevented systematic analysis of RAP identities, dynamics, and functions. We have developed a label-free methodology called RAPIDASH to enrich ribosomes and RAPs from any sample. We applied RAPIDASH to mouse embryonic tissues and identified hundreds of potential RAPs, including DHX30 and LLPH, two forebrain RAPs important for neurodevelopment. We identified a critical role of LLPH in neural development linked to the translation of genes with long coding sequences. In addition, we showed RAPIDASH can identify ribosome changes in cancer cells. Finally, we characterized ribosome composition remodeling during immune cell activation and observed extensive changes post-stimulation. RAPIDASH has therefore enabled the discovery of RAPs in multiple cell types, tissues, and stimuli and is adaptable to characterize ribosome remodeling in several contexts.
Published in
RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages
Susanto TT, Hung V, Levine AG et al. · Molecular cell 2024 · PMID 39260367 · doi:10.1016/j.molcel.2024.08.023
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Direct links to NCBI, no account and no request form: the whole study as GSE274188_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 12 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1145378 and SRA study SRP524765. Searching any of these in the dataset finder brings you back here.

Study design
3 × H1 hiN D14, LLPHNterm/Nterm, Ribo-seq, vs 3 × H1 hiN D14, LLPHwt/wt, Ribo-seq, vs 3 × H1 hiN D14, LLPHNterm/Nterm, RNAseq, vs 3 × H1 hiN D14, LLPHwt/wt, RNAseq,

Supports a between-group comparison across 12 samples.

4 replicated groups read from 12 sample titles; they account for 12 of them. Check it against the sample list below before relying on it.

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