GEO series

Integrated RNA, translation, and proteome profiling reveals midbody remnants as selectively organized signaling vesicles

GSE305263 Homo sapiens Other; Expression profiling by high throughput sequencing 42 samples Submitted 2026/08/04 Platform GPL20301Platform GPL34284

Summary

This study investigates the differences in mRNA payloads between midbody remnants (MBRs) and whole cell of origin for six cell types, in order to determine type-specific and common enriched genes in MBRs. RNA-seq was performed by the Skop lab for all six cell types. Ribo-seq was performed by the Iwasaki lab on one of the cell types to assess translational differences. Data include raw FASTQ files, processed count tables, and metadata describing each sample.

This dataset

Open GSE305263 in finder → Find more human datasets →

Download

Direct links to NCBI, no account and no request form: the whole study as GSE305263_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 42 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1304953. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

13 conditions, mostly in triplicate

human_esc_cell_rna ×3 human_esc_mbr_rna ×3 human_hela_cell_rna ×3 human_hela_mbr_rna ×3 human_hepg2_cell_rna ×3 human_hepg2_mbr_rna ×3 human_ipsc_cell_rna ×3 human_ipsc_mbr_rna ×3 +6 more

Supports a between-group comparison across 39 samples.

13 replicated groups read from the first 40 of 42 sample titles; they account for 39 of them. Check it against the sample list below before relying on it.

Similar datasets

Search all human datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.