← BioTransfer GEO Dataset Finder
GEO series

TH-positive pulmonary nerve fiber-epithelial interaction promotes adipose thermogenesis in male mice [C22_RNA-seq]

GSE342447 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/08/08 Platform GPL34328
Summary
Cold exposure activates sympathetic neurons in adipose tissue to maintain thermal homeostasis. However, whether sympathetic activation in non-adipose tissues contributes to cross-organ regulation of adipose thermogenesis remains unclear. Here, we demonstrate the activation of TH-positive pulmonary nerve fibers (TPNFs) by cold exposure, which further promotes adipose thermogenesis. Specifically, cold exposure activates TPNFs that innervate bronchial Club cells in the lung and leads to a reduction in Secretoglobin family 3A member 1 (SCGB3A1) expression via Adrenergic Receptor β2 (ADRB2). SCGB3A1, a Club cell-specific secretory factor, binds the choline transporter SLC44A2 on adipocytes to restrict choline uptake and impair thermogenic activation. Meanwhile, TPNFs activation induces NGF secretion from Club cells, which in turn strengthens TPNFs innervation of Club cells in a feedforward loop. Importantly, the stimulation of this mechanism via tracheal infusion of the ADRB2 agonist norepinephrine (NE) enhances thermogenesis in mice.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE342447_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1507884 and SRA study SRP724342. Searching any of these in the dataset finder brings you back here.

Study design
2 × C22 cells, vehicle control, 3h, vs 2 × C22 cells, NE treatment (10 μM), 3h,

Supports a between-group comparison across 4 samples.

2 replicated groups read from 4 sample titles; they account for 4 of them. Check it against the sample list below before relying on it.

Samples in this study
Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.