GEO series
Multi-organ proteomic atlas of obesity regression in male mice
GSE338985
Mus musculus
Expression profiling by high throughput sequencing
94 samples
Submitted 2026/07/17
Platform GPL24247
Summary
Obesity drives systemic metabolic dysfunction, yet how the body adapts and recovers at a system-wide level remains unclear. Here we generate a multi-organ proteomic atlas of diet-induced obesity and its regression in male mice. Using a standardized, semi-automated sample preparation workflow, we quantify 12,936 unique proteins across 15 organs and 4 timepoints. We find proteomic changes to be highly tissue-dependent with a small number of proteins displaying shared changes across multiple tissues. While proteomes of most tissues revert to lean levels after weight loss, white adipose tissue retains strong phagocytic and inflammatory responses, and brain, kidney, bone, thymus, and spleen display delayed obesity-induced alterations. We map the adipose tissue-specific immune regulators using ligand-target inference and show reduced expression of proteasomal subunits in brown adipose tissue, resulting in stalled ubiquitin turnover. Finally, we make all datasets open access and create an interactive webtool to facilitate further community-driven discovery.
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE338985_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 94 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1496755 and SRA study SRP718849. Searching any of these in the dataset finder brings you back here.
Study design
40 conditions, each sampled once — no replicated groups
Read from the first 40 of 94 sample titles: 40 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9886024 epididymal white adipose tissue, Long-term lean control, biological replicate 1, mouse SMA_3756
- GSM9886025 epididymal white adipose tissue, Long-term lean control, biological replicate 2, mouse SMA_3757
- GSM9886026 epididymal white adipose tissue, Long-term lean control, biological replicate 3, mouse SMA_3758
- GSM9886027 epididymal white adipose tissue, Long-term lean control, biological replicate 4, mouse SMA_3708
- GSM9886028 epididymal white adipose tissue, Long-term lean control, biological replicate 5, mouse SMA_3709
- GSM9886029 epididymal white adipose tissue, Long-term lean control, biological replicate 6, mouse SMA_3710
- GSM9886030 epididymal white adipose tissue, Long-term obesity regression, biological replicate 1, mouse SMA_3747
- GSM9886031 epididymal white adipose tissue, Long-term obesity regression, biological replicate 2, mouse SMA_3748
- GSM9886032 epididymal white adipose tissue, Long-term obesity regression, biological replicate 3, mouse SMA_3749
- GSM9886033 epididymal white adipose tissue, Long-term obesity regression, biological replicate 4, mouse SMA_3723
- GSM9886034 epididymal white adipose tissue, Long-term obesity regression, biological replicate 5, mouse SMA_3724
- GSM9886035 epididymal white adipose tissue, Long-term obesity regression, biological replicate 6, mouse SMA_3725
- GSM9886036 epididymal white adipose tissue, Medium-term lean control, biological replicate 1, mouse SMA_3753
- GSM9886037 epididymal white adipose tissue, Medium-term lean control, biological replicate 2, mouse SMA_3754
- GSM9886038 epididymal white adipose tissue, Medium-term lean control, biological replicate 3, mouse SMA_3755
- GSM9886039 epididymal white adipose tissue, Medium-term lean control, biological replicate 4, mouse SMA_3705
- GSM9886040 epididymal white adipose tissue, Medium-term lean control, biological replicate 5, mouse SMA_3706
- GSM9886041 epididymal white adipose tissue, Medium-term lean control, biological replicate 6, mouse SMA_3707
- GSM9886042 epididymal white adipose tissue, Medium-term obesity regression, biological replicate 1, mouse SMA_3765
- GSM9886043 epididymal white adipose tissue, Medium-term obesity regression, biological replicate 2, mouse SMA_3766
- GSM9886044 epididymal white adipose tissue, Medium-term obesity regression, biological replicate 3, mouse SMA_3767
- GSM9886045 epididymal white adipose tissue, Medium-term obesity regression, biological replicate 4, mouse SMA_3699
- GSM9886046 epididymal white adipose tissue, Medium-term obesity regression, biological replicate 5, mouse SMA_3700
- GSM9886047 epididymal white adipose tissue, Medium-term obesity regression, biological replicate 6, mouse SMA_3701
- GSM9886048 epididymal white adipose tissue, Short-term lean control, biological replicate 1, mouse SMA_3762
- GSM9886049 epididymal white adipose tissue, Short-term lean control, biological replicate 2, mouse SMA_3763
- GSM9886050 epididymal white adipose tissue, Short-term lean control, biological replicate 3, mouse SMA_3764
- GSM9886051 epididymal white adipose tissue, Short-term lean control, biological replicate 4, mouse SMA_3717
- GSM9886052 epididymal white adipose tissue, Short-term lean control, biological replicate 5, mouse SMA_3718
- GSM9886053 epididymal white adipose tissue, Short-term lean control, biological replicate 6, mouse SMA_3719
- GSM9886054 epididymal white adipose tissue, Short-term obesity regression, biological replicate 1, mouse SMA_3744
- GSM9886055 epididymal white adipose tissue, Short-term obesity regression, biological replicate 2, mouse SMA_3745
- GSM9886056 epididymal white adipose tissue, Short-term obesity regression, biological replicate 3, mouse SMA_3746
- GSM9886057 epididymal white adipose tissue, Short-term obesity regression, biological replicate 4, mouse SMA_3711
- GSM9886058 epididymal white adipose tissue, Short-term obesity regression, biological replicate 5, mouse SMA_3712
- GSM9886059 epididymal white adipose tissue, Short-term obesity regression, biological replicate 6, mouse SMA_3713
- GSM9886060 epididymal white adipose tissue, Lean control at obesity time point, biological replicate 1, mouse SMA_3783
- GSM9886061 epididymal white adipose tissue, Lean control at obesity time point, biological replicate 2, mouse SMA_3784
- GSM9886062 epididymal white adipose tissue, Lean control at obesity time point, biological replicate 3, mouse SMA_3785
- GSM9886063 epididymal white adipose tissue, Lean control at obesity time point, biological replicate 4, mouse SMA_3726
+ 54 more — browse all 94 samples with per-sample file links →
Similar datasets
- GSE314817 Multi-tissue, multi-time-point transcriptomic atlas of aging in mice and rats [mouse] 2432 samples
- GSE304862 Semaglutide and exercise synergy in obesity: preserving muscle mass and uncovering organ crosstalk 209 samples
- GSE341948 Multi-tissue transcriptomic landscape reveals synergistic mechanisms of exercise and GLP-1 agonist in ameliorating diabetic phenotypes in db/db mice 12 samples
- GSE329539 Hypothalamic transcriptomic response to High-Fat Diet induced obesity: modulatory effects of Resveratrol, Curcumin, and Aged Garlic Extract. 12 samples
- GSE293709 Transcriptome analysis in the prefrontal cortex of different ASD mouse models treated with drug 1565 samples
- GSE294832 Modular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice [part10] 1000 samples
- GSE326100 scRNA-seq of mouse cochlea organoid cells 768 samples
- GSE331176 Phagosome-mediated activation of STING by purine and pyrimidine-based bacterial cyclic dinucleotides 380 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.