GEO series
Coordinating catalytic and non-canonical functions of EZH2 sensitizes tumors to CAR-T cell therapy
GSE323364
Homo sapiens
Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing
42 samples
Submitted 2026/05/21
Platform GPL20301
Summary
This study investigates the dual roles of EZH2 (catalytic and non-canonical) in regulating tumor cell adhesion and sensitivity to CAR-T cell therapy. We performed RNA-seq and CUT&Tag-seq (anti-EZH2) on three human triple-negative breast cancer cell lines (MDA-MB-436, MDA-MB-468, HCC1806) treated with the EZH2 inhibitor EPZ-6438. Additionally, RNA-seq was performed on MDA-MB-468 cells with EZH2 knockdown to dissect the EZH2-dependent transcriptomic underlying tumor cell adhesion and CAR-T therapy response.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE323364_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 42 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1432532 and SRA study SRP681163. Searching any of these in the dataset finder brings you back here.
Study design
14 conditions, mostly in triplicate
RNA-seq of MDA-MB-468 EPZ-6438 ×3
RNA-seq of MDA-MB-468 DMSO ×3
CUT&Tag of MDA-MB-468 EPZ-6438 ×3
CUT&Tag of MDA-MB-468 DMSO ×3
RNA-seq of MDA-MB-436 EPZ-6438 ×3
RNA-seq of MDA-MB-436 DMSO ×3
CUT&Tag of MDA-MB-436 EPZ-6438 ×3
CUT&Tag of MDA-MB-436 DMSO ×3
+6 more
Supports a between-group comparison across 40 samples.
14 replicated groups read from the first 40 of 42 sample titles; they account for 40 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9564358 RNA-seq of MDA-MB-468 EPZ-6438 rep1
- GSM9564359 RNA-seq of MDA-MB-468 DMSO rep1
- GSM9564360 RNA-seq of MDA-MB-468 EPZ-6438 rep2
- GSM9564361 RNA-seq of MDA-MB-468 DMSO rep2
- GSM9564362 RNA-seq of MDA-MB-468 EPZ-6438 rep3
- GSM9564363 RNA-seq of MDA-MB-468 DMSO rep3
- GSM9564364 CUT&Tag of MDA-MB-468 EPZ-6438 rep1
- GSM9564365 CUT&Tag of MDA-MB-468 DMSO rep1
- GSM9564366 CUT&Tag of MDA-MB-468 EPZ-6438 rep2
- GSM9564367 CUT&Tag of MDA-MB-468 DMSO rep2
- GSM9564368 CUT&Tag of MDA-MB-468 EPZ-6438 rep3
- GSM9564369 CUT&Tag of MDA-MB-468 DMSO rep3
- GSM9564370 RNA-seq of MDA-MB-436 EPZ-6438 rep1
- GSM9564371 RNA-seq of MDA-MB-436 DMSO rep1
- GSM9564372 RNA-seq of MDA-MB-436 EPZ-6438 rep2
- GSM9564373 RNA-seq of MDA-MB-436 DMSO rep2
- GSM9564374 RNA-seq of MDA-MB-436 EPZ-6438 rep3
- GSM9564375 RNA-seq of MDA-MB-436 DMSO rep3
- GSM9564376 CUT&Tag of MDA-MB-436 EPZ-6438 rep1
- GSM9564377 CUT&Tag of MDA-MB-436 DMSO rep1
- GSM9564378 CUT&Tag of MDA-MB-436 EPZ-6438 rep2
- GSM9564379 CUT&Tag of MDA-MB-436 DMSO rep2
- GSM9564380 CUT&Tag of MDA-MB-436 EPZ-6438 rep3
- GSM9564381 CUT&Tag of MDA-MB-436 DMSO rep3
- GSM9564382 RNA-seq of HCC-1806 EPZ-6438 rep1
- GSM9564383 RNA-seq of HCC-1806 DMSO rep1
- GSM9564384 RNA-seq of HCC-1806 EPZ-6438 rep2
- GSM9564385 RNA-seq of HCC-1806 DMSO rep2
- GSM9564386 RNA-seq of HCC-1806 EPZ-6438 rep3
- GSM9564387 RNA-seq of HCC-1806 DMSO rep3
- GSM9564388 CUT&Tag of HCC-1806 EPZ-6438 rep1
- GSM9564389 CUT&Tag of HCC-1806 DMSO rep1
- GSM9564390 CUT&Tag of HCC-1806 EPZ-6438 rep2
- GSM9564391 CUT&Tag of HCC-1806 DMSO rep2
- GSM9564392 CUT&Tag of HCC-1806 EPZ-6438 rep3
- GSM9564393 CUT&Tag of HCC-1806 DMSO rep3
- GSM9564394 RNAseq_468_shEZH2_rep1
- GSM9564395 RNAseq_468_shNC_rep1
- GSM9564396 RNAseq_468_shEZH2_rep2
- GSM9564397 RNAseq_468_shNC_rep2
+ 2 more — browse all 42 samples with per-sample file links →
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