GEO series
Robust effector memory features of human T-bet-hi B cells induced by repeated mRNA vaccination
GSE315668
Homo sapiens
Other; Expression profiling by high throughput sequencing
42 samples
Submitted 2026/01/13
Platform GPL16791
Summary
The heterogeneity of memory B cells in response to repetitive cognate antigen challenges remains to be fully elucidated. Here we identified a transcriptionally distinct cluster of T-bethi B cells, among SARS-CoV-2 RBD-specific B cells in PBMCs from healthy individuals vaccinated with the BNT162b2 SARS-CoV-2 mRNA vaccine. Our findings indicate that T-bethi B cells can be defined by a combination of CD11c and FcRL5 receptors, and are distinguished by distinct gene regulatory networks associated with effector functions. Notably, these T-bethi B cells were affinity-matured and exhibited rapid differentiation into antibody-secreting cells (ASCs) producing neutralizing antibodies comparable to classical memory B cells, underscoring their role in early recall responses. Taken together, these findings illuminate the potent effector memory roles of T-bethi B cells in adaptive immunity following vaccinations.
Published in
Lee J, Son S, Chung Y et al.
· iScience 2026
· PMID 41716987
· doi:10.1016/j.isci.2026.114804
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE315668_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 42 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1398655 and SRA study SRP659955. Searching any of these in the dataset finder brings you back here.
Study design
20 conditions, mostly in duplicate
GEX,Bcells,timepoint1,tube ×2
GEX,Bcells,timepoint2,tube ×2
GEX,Bcells,timepoint3,tube ×2
GEX,Bcells,timepoint4,tube ×2
GEX,Bcells,timepoint5,tube ×2
GEX,Bcells,timepoint6,tube ×2
GEX,Bcells,timepoint7,tube ×2
FB,Bcells,timepoint1,tube ×2
+12 more
Supports a between-group comparison across 40 samples.
20 replicated groups read from the first 40 of 42 sample titles; they account for 40 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9434288 GEX,Bcells,timepoint1,tube1
- GSM9434289 GEX,Bcells,timepoint1,tube2
- GSM9434290 GEX,Bcells,timepoint2,tube1
- GSM9434291 GEX,Bcells,timepoint2,tube2
- GSM9434292 GEX,Bcells,timepoint3,tube1
- GSM9434293 GEX,Bcells,timepoint3,tube2
- GSM9434294 GEX,Bcells,timepoint4,tube1
- GSM9434295 GEX,Bcells,timepoint4,tube2
- GSM9434296 GEX,Bcells,timepoint5,tube1
- GSM9434297 GEX,Bcells,timepoint5,tube2
- GSM9434298 GEX,Bcells,timepoint6,tube1
- GSM9434299 GEX,Bcells,timepoint6,tube2
- GSM9434300 GEX,Bcells,timepoint7,tube1
- GSM9434301 GEX,Bcells,timepoint7,tube2
- GSM9434302 FB,Bcells,timepoint1,tube1
- GSM9434303 FB,Bcells,timepoint1,tube2
- GSM9434304 FB,Bcells,timepoint2,tube1
- GSM9434305 FB,Bcells,timepoint2,tube2
- GSM9434306 FB,Bcells,timepoint3,tube1
- GSM9434307 FB,Bcells,timepoint3,tube2
- GSM9434308 FB,Bcells,timepoint4,tube1
- GSM9434309 FB,Bcells,timepoint4,tube2
- GSM9434310 FB,Bcells,timepoint5,tube1
- GSM9434311 FB,Bcells,timepoint5,tube2
- GSM9434312 FB,Bcells,timepoint6,tube1
- GSM9434313 FB,Bcells,timepoint6,tube2
- GSM9434314 FB,Bcells,timepoint7,tube1
- GSM9434315 FB,Bcells,timepoint7,tube2
- GSM9434316 BCR,Bcells,timepoint1,tube1
- GSM9434317 BCR,Bcells,timepoint1,tube2
- GSM9434318 BCR,Bcells,timepoint2,tube1
- GSM9434319 BCR,Bcells,timepoint2,tube2
- GSM9434320 BCR,Bcells,timepoint3,tube1
- GSM9434321 BCR,Bcells,timepoint3,tube2
- GSM9434322 BCR,Bcells,timepoint4,tube1
- GSM9434323 BCR,Bcells,timepoint4,tube2
- GSM9434324 BCR,Bcells,timepoint5,tube1
- GSM9434325 BCR,Bcells,timepoint5,tube2
- GSM9434326 BCR,Bcells,timepoint6,tube1
- GSM9434327 BCR,Bcells,timepoint6,tube2
+ 2 more — browse all 42 samples with per-sample file links →
Similar datasets
- GSE329935 DNA repair drives cisplatin-induced neuronal death 28 samples
- GSE294296 Cutaneous lupus features specialized stromal niches and altered retroelement expression 16 samples
- GSE285515 NKG2D blockade impairs cytotoxic CD8+ tissue resident memory T cell accumulation and attenuates severity of chronic lung allograft dysfunction after transplantation 15 samples
- GSE284343 Eighteen-year survival after GD2-directed Chimeric Antigen Receptor-Modified Immune Effector Cell Treatment for Neuroblastoma 12 samples
- GSE248132 LARP6 promotes carcinogenic features of triple negative breast cancer cell line by directly modulating alternative splicing of associated genes 10 samples
- GSE270126 Divergent medulloblastoma chromatin states disclose KDM2B as a selective dependency. 219 samples
- GSE300844 Deciphering the functional impact of Alzheimer’s Disease-associated variants in resting and proinflammatory immune cells 192 samples
- GSE310353 In situ multi-modal characterization of pancreatic cancer reveals tumor cell identity as a defining factor of the surrounding microenvironment 116 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.