GEO series
The Cd4+ T cell population Partners with Tpex CD8 T cells to mediate Antitumor Immunity in the Tumor Microenvironment.[PB cohort2]
GSE308745
Homo sapiens
Expression profiling by high throughput sequencing; Other
87 samples
Submitted 2026/02/05
Platform GPL16791Platform GPL28038Platform GPL24676
Summary
The priming, expansion, and function of CD8⁺ T cells are helped by CD4⁺ T cells via dendritic cells. Precursor exhausted T cells (Tpex) maintain self-renewal and supply cytotoxic CD8⁺ T cells in the tumor microenvironment (TME), but the identity of their CD4⁺ T-cell partners remains unclear. Here, we perform scRNA-seq, scTCR-seq, and mass cytometry analysis on peripheral blood, tumor, and lymph nodes derived from lung cancer patients and identify a population of IL-7Rhigh CCR6⁺ Th1-like CD4⁺ T cells, namedTh7R, that is numerically and spatially partnered with Tpex cells. Th7R cells express lymphotoxin-β and CXCL13, correlate with high endothelial venules, and co-localize with Tpex in tertiary lymphoid structures. Furthermore, Th7R cell abundance correlates with Tpex numbers in the TME and lymph nodes, and adoptive transfer of Th7R increases Tpex in a preclinical mouse model of skin cancer. In lung cancer patients, Th7R and Tpex in TME are associated with better response to neoadjuvant PD-1 blockade therapy. Thus, these results suggest that Th7R cells act as partners of Tpex CD8 T cells to sustain antitumor T-cell immunity.
Published in
Takei S, Yamasaki S, Yamaguchi O et al.
· Nature communications 2026
· PMID 41904165
· doi:10.1038/s41467-026-71161-0
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE308745_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 87 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1332649 and SRA study SRP624803. Searching any of these in the dataset finder brings you back here.
Study design
40 conditions, each sampled once — no replicated groups
Read from the first 40 of 87 sample titles: 40 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9252455 RE52, PBMC before treatment, scRNA-seq
- GSM9252456 RE52, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252457 RE52, PBMC before treatment, scTCR-seq
- GSM9252458 RE55, PBMC before treatment, scRNA-seq
- GSM9252459 RE55, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252460 RE55, PBMC before treatment, scTCR-seq
- GSM9252461 RE89, PBMC before treatment, scRNA-seq
- GSM9252462 RE89, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252463 RE89, PBMC before treatment, scTCR-seq
- GSM9252464 RE97, PBMC before treatment, scRNA-seq
- GSM9252465 RE97, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252466 RE97, PBMC before treatment, scTCR-seq
- GSM9252467 RE106, PBMC before treatment, scRNA-seq
- GSM9252468 RE106, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252469 RE106, PBMC before treatment, scTCR-seq
- GSM9252470 RE159, PBMC before treatment, scRNA-seq
- GSM9252471 RE159, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252472 RE159, PBMC before treatment, scTCR-seq
- GSM9252473 RE187, PBMC before treatment, scRNA-seq
- GSM9252474 RE187, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252475 RE187, PBMC before treatment, scTCR-seq
- GSM9252476 RE188, PBMC before treatment, scRNA-seq
- GSM9252477 RE188, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252478 RE188, PBMC before treatment, scTCR-seq
- GSM9252479 I439, PBMC before treatment, scRNA-seq
- GSM9252480 I439, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252481 I439, PBMC before treatment, scTCR-seq
- GSM9252482 I489, PBMC before treatment, scRNA-seq
- GSM9252483 I489, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252484 I489, PBMC before treatment, scTCR-seq
- GSM9252485 I554, PBMC before treatment, scRNA-seq
- GSM9252486 I554, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252487 I554, PBMC before treatment, scTCR-seq
- GSM9252488 I559, PBMC before treatment, scRNA-seq
- GSM9252489 I559, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252490 I559, PBMC before treatment, scTCR-seq
- GSM9252491 I664, PBMC before treatment, scRNA-seq
- GSM9252492 I664, PBMC before treatment, Feature Barcoding(ADT)
- GSM9252493 I664, PBMC before treatment, scTCR-seq
- GSM9252494 G2, PBMC before treatment, scRNA-seq
+ 47 more — browse all 87 samples with per-sample file links →
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