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Profiling the RNA-Binding Proteome of KSHV-Infected Cells Uncovers a Viral Strategy for Hijacking Host Signaling via RNA-Dependent Interactions.

GSE308077 Homo sapiens Expression profiling by high throughput sequencing 18 samples Submitted 2026/08/07 Platform GPL34281
Summary
Viruses encode RNA-binding proteins (RBPs) to manipulate host processes and promote infection. However, due to limited genomic space, many viral proteins are multifunctional and may engage RNA through non-canonical domains, making their identification difficult using traditional sequence-based approaches. Kaposi’s sarcoma-associated herpesvirus (KSHV) is the etiological agent of many AIDS-associated cancers. Here, leveraging an unbiased proteomic approach we identified all RBPs in KSHV-infected primary effusion lymphoma (PEL) cells, uncovering 7 previously unrecognized KSHV-encoded RBPs. Among these is viral Interferon Regulatory Factor 1 (vIRF1), which we found is both necessary and sufficient to activate oncogenic RAS/MAPK signaling through an RNA-dependent interaction with host G3BP proteins. Importantly, a KSHV vIRF1 mutant lacking the ability to bind RNA exhibits a B cell-specific replication defect, highlighting the essential role of RNA interactions in viral gene regulation and pathogenesis. These findings reveal a novel strategy by which KSHV hijacks RNA-mediated signaling to support its lifecycle.
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Direct links to NCBI, no account and no request form: the whole study as GSE308077_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 18 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1328701 and SRA study SRP620475. Searching any of these in the dataset finder brings you back here.

Study design
6 conditions, mostly in triplicate
BJAB-BAC16 WT latent ×3 BJAB-BAC16 REV latent ×3 BJAB-BAC16 AAA latent ×3 BJAB-BAC16 WT lytic ×3 BJAB-BAC16 REV lytic ×3 BJAB-BAC16 AAA lytic ×3

Supports a between-group comparison across 18 samples.

6 replicated groups read from 18 sample titles; they account for 18 of them. Check it against the sample list below before relying on it.

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