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Side- and disease-dependent changes in human aortic valve cell population and transcriptomic heterogeneity determined by single-cell RNA sequencing

GSE220774 Homo sapiens Expression profiling by high throughput sequencing 15 samples Submitted 2024/12/01 Platform GPL24676
Summary
Calcific aortic valve disease develops preferentially on the fibrosa side while the ventricularis side remains relatively spared with unknown mechanisms. We hypothesized that the fibrosa is prone to the disease due to side-dependent differences in transcriptomic patterns and cell phenotypes. To test this hypothesis, we performed single-cell RNA-sequencing using a new method to collect endothelial-enriched samples from either the fibrosa or ventricularis of freshly-obtained human AV leaflets from 5 donors ranging from non-diseased to fibrocalcific stages.
Published in
Side- and Disease-Dependent Changes in Human Aortic Valve Cell Population and Transcriptomic Heterogeneity Determined by Single-Cell RNA Sequencing
Villa-Roel N, Park C, Andueza A et al. · Genes 2024 · PMID 39766890 · doi:10.3390/genes15121623
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Direct links to NCBI, no account and no request form: the whole study as GSE220774_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 15 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA911302 and SRA study SRP412622. Searching any of these in the dataset finder brings you back here.

Study design
13 conditions, each sampled once — no replicated groups
Donor #4 Leftove ×3

Read from 15 sample titles: 13 distinct titles with little repetition. Check it against the sample list below before relying on it.

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