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Histone chaperon ASF1B recruits distinct epigenetic factors in regulation of histone variant H3.3 during the fetal and adult erythropoiesis II

GSE285838 Mus musculus; Homo sapiens Expression profiling by high throughput sequencing 26 samples Submitted 2026/05/15 Platform GPL34281Platform GPL34328
Summary
To gain insight into ASF1A, ASF1B and HIRA function during erythropoiesis, RNA-seq was performed in murine erythroleukemia (MEL) cells, human umbilical cord-derived progenitor erythroid 2 (HUDEP2) cells and human CD34+ erythroid progenitor cells. Notably, depletion of ASF1A, ASF1B and HIRA with/without DMSO induction significantly reduced the expression of genes associated with erythroid differentiation. Meanwhile, depletion of ASF1A and ASF1B in HUDEP2 cells as well as depletion of ASF1B in CD34+ cells significantly reduced the expression of genes associated with erythroid differentiation.
Published in
ASF1B promotes erythropoiesis by regulating the establishment and enrichment of H3.3 nucleosomes
Liu J, Song X, Zhou L et al. · Nucleic acids research 2026 · PMID 42100853 · doi:10.1093/nar/gkag447
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Direct links to NCBI, no account and no request form: the whole study as GSE285838_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 26 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1206179 and SRA study SRP559498. Searching any of these in the dataset finder brings you back here.

Study design
11 conditions, mostly in duplicate
control uninduce ×2 ASF1A KO uninduce ×2 ASF1B KO uninduce ×2 HIRA KO uninduce ×2 control induce ×2 ASF1A KO induce ×2 ASF1B KO induce ×2 HIRA KO induce ×2 +7 more

Supports a between-group comparison across 22 samples.

11 replicated groups read from 26 sample titles; they account for 22 of them. Check it against the sample list below before relying on it.

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