GEO series
Clinical and Immunological Outcomes after Randomized Trial of Baked Milk Oral Immunotherapy for Milk Allergy
GSE280063
Homo sapiens
Expression profiling by high throughput sequencing; Other
24 samples
Submitted 2025/01/13
Platform GPL24676
Summary
BACKGROUND. Cow’s milk allergy (CMA) is the most common food allergy in young children. Treatment with oral immunotherapy (OIT) has shown efficacy but also high rates of adverse reactions. We sought to determine if baked milk (BM) OIT could reduce adverse reactions while still inducing desensitization, and to identify immunological correlates of successful BMOIT. METHODS. This phase II, randomized trial evaluated the safety and efficacy of BMOIT in children age 3-18 years. After the initial placebo-controlled first year of treatment, placebo-treated participants crossed over to active BMOIT for year two. Double-blind, placebo-controlled food challenges (DBPCFC) were conducted to BM after year one and to both BM and unheated milk (UM) after year two. IgG/IgE antibodies were measured along with cow’s milk (CM)-specific CD4 memory T cell populations, profiled using flow cytometry and scRNA-Seq. RESULTS. Twenty-one of 30 (70%) reached the primary endpoint of tolerating 4044mg of BM protein at month-24, and 11/30 tolerated ≥2000mg of UM. Dosing symptoms were common, but >98% were mild with no severe reactions. Immunological changes associated with desensitization included increased CM IgG4, CM+ FOXP3+ cells, and Tregs and corresponding decreases in CM IgE, CM+ Th2A cells, and CD154+ cells. T cell and antibody measurements were combined to build a model that predicted UM oral food challenge (OFC) outcomes. CONCLUSION. BMOIT was well tolerated and induced a substantial level of desensitization to baked and unheated milk. This desensitization corresponded to significant redistribution within antigen specific antibody and T cell compartments that provide novel insight into the mechanistic changes that occur with OIT treatment.
Published in
Dantzer JA, Lewis SA, Psoter KJ et al.
· JCI insight 2025
· PMID 39782691
· doi:10.1172/jci.insight.184301
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE280063_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 24 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1176124. Searching any of these in the dataset finder brings you back here.
Study design
24 conditions, each sampled once — no replicated groups
Read from 24 sample titles: 24 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM8586724 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube1A
- GSM8586725 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube1A
- GSM8586726 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube1B
- GSM8586727 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube1B
- GSM8586728 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube2A
- GSM8586729 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube2A
- GSM8586730 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube2B
- GSM8586731 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube2B
- GSM8586732 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube3A
- GSM8586733 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube3A
- GSM8586734 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube3B
- GSM8586735 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube3B
- GSM8586736 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube4A
- GSM8586737 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube4A
- GSM8586738 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube4B
- GSM8586739 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube4B
- GSM8586740 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube5A
- GSM8586741 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube5A
- GSM8586742 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube5B
- GSM8586743 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube5B
- GSM8586744 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube6A
- GSM8586745 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube6A
- GSM8586746 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, hashtag, tube6B
- GSM8586747 CD4 memory T cells, cow milk allergic and non-allergic pediatric subjects, Gene expression, tube6B
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