GEO series
Molecular characterization of Wilms' Tumor: insights from single nucleus analystics on Wilms' Tumor development and oncogenesis
GSE273662
Homo sapiens
Expression profiling by high throughput sequencing
14 samples
Submitted 2026/08/08
Platform GPL24676
Summary
Wilms tumors are known to arise from and maintain features of the embryonic kidney associated with a surprisingly heterogenous genetic and molecular landscape. To further investigate both intra-tumor and inter-tumor variability, single nucleus RNA-sequencing (snRNA-seq) was performed on favorable histology Wilms tumors capturing blastema, epithelial, and stromal components, which were analyzed in comparison to a normal kidney reference atlas established from kidney samples (including patient-matched adjacent kidney) as well as publicly available single-cell RNA-seq from 18-week human fetal kidney. The transcriptomic profiles from Wilms tumor nuclei were shown to recapitulate its known triphasic histology, with similar gene expression signatures corresponding to nephron progenitor cells as well epithelial and stromal cells from human fetal kidneys. Tumors additionally show a high percentage of cycling cells in the G2/S phase, including tumor samples with blastemal cells both resembling “uninduced” nephron progenitors as well as tumor samples with blastema expressing more “differentiated” signatures of early proximal tubules, podocytes, as well as stromal/muscle genes. Furthermore, pathway enrichment analyses in the blastemal components show nephron/kidney epithelial development as expected, but interestingly also show neuronal/axon signatures across the majority of tumor samples. Additionally, enriched pathways in a subset of the tumors include WNT and TGF-beta signaling in the non-cycling blastema and polycomb repressive complex in cycling blastemal cells, suggesting that Wilms tumor blastema not only shows significant heterogeneity in its differentiation state/trajectory but also in its potential biological pathways/drivers.
Published in
Adam M, Drake KA, Pode-Shakked N et al.
· JCI insight 2026
· PMID 42262873
· doi:10.1172/jci.insight.188078
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE273662_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 14 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1142582 and SRA study SRP523415. Searching any of these in the dataset finder brings you back here.
Study design
9 × T vs 5 × K
Supports a between-group comparison across 14 samples.
2 replicated groups read from 14 sample titles; they account for 14 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM8434760 T5
- GSM8434761 K5
- GSM8434762 K4
- GSM8434763 T4
- GSM8434764 T1
- GSM8434765 K1
- GSM8434766 K2
- GSM8434767 T2
- GSM8434768 K3
- GSM8434769 T3
- GSM8434770 T8
- GSM8434771 T9
- GSM8434772 T7
- GSM8434773 T6
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