GEO series
Longitudinal single-cell transcriptomics of monocytes, Tfh and gd Tcells following a control human malaria infection.
GSE253661
Homo sapiens
Expression profiling by high throughput sequencing; Other
16 samples
Submitted 2025/10/05
Platform GPL21697
Summary
Malaria infection drives tolerogenic cell responses which protects from high inflammation and immunopathogenesis but reduces parasite control and adaptive immunity. Here, we comprehensively map responses across innate and adaptive cells. The results of this Controlled Human Malaria Infection study are presented in three independent publications. Blood samples from 4 donors were collected prior to infection (day 0), at peak infection (day 8) and 14 or 15 and 27-36 days (end of study, EOS) after inoculation (in analyses these time points are grouped as 0, 8, 14/15 and EOS). Participants were healthy malaria naïve adults with no prior exposure to malaria or residence in malaria-endemic regions. For trials NCT02867059, NCT02783833, NCT02431637 all participants received the same study drug (no-randomization) and 3D7-strain parasites. For NCT02431650, participants were randomized 1:1 to receive study drug or control and 3D7-strain parasites. Samples used here were from samples opportunistically collected from volunteers who consented to donate blood for immunological studies within the parent clinical trial. As such, no sample size estimation was performed for this immunology study.
Published in
Soon MS, Oyong DA, Dooley NL et al.
· JCI insight 2025
· PMID 41118256
· doi:10.1172/jci.insight.196828
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE253661_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 16 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1066724 and SRA study SRP484709. Searching any of these in the dataset finder brings you back here.
Study design
16 conditions, each sampled once — no replicated groups
Read from 16 sample titles: 16 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM8024711 monocyte day 0 pre-infection
- GSM8024712 monocyte day 8 post-infection
- GSM8024713 monocyte day 16 post-infection
- GSM8024714 monocyte day 36 post-infection
- GSM8024715 tfh day 0 pre-infection
- GSM8024716 tfh day 8 post-infection
- GSM8024717 tfh day 16 post-infection
- GSM8024718 tfh day 36 post-infection
- GSM8024719 gd day 0 pre-infection
- GSM8024720 gd day 8 post-infection
- GSM8024721 gd day 16 post-infection
- GSM8024722 gd day 36 post-infection
- GSM8437028 TCR_tfh day 0 pre-infection
- GSM8437029 TCR_tfh day 8 post-infection
- GSM8437030 TCR_tfh day 16 post-infection
- GSM8437031 TCR_tfh day 36 post-infection
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