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Development-inspired approach for the derivation of functional and transplantable salivary gland organoids from human pluripotent stem cells [RNA-Seq]

GSE342790 Homo sapiens Expression profiling by high throughput sequencing 16 samples Submitted 2026/08/07 Platform GPL24676
Summary
Salivary gland (SG) hypofunction significantly affects the life quality of patients with hyposalivation. Current treatment options for these conditions are limited and often result in undesirable side effects. To address this issue, we developed a novel and developmentally inspired strategy to derive salivary gland epithelial progenitor (SGEP) cells from human pluripotent stem cells (PSC). Then we employed SGEP cells derived from human iPSC with a SOX10::GFP reporter to engineer three-dimensional lumenized SG organoids, with well-defined lumens and secretory function, reminiscent of human salivary glands. Upon transplantation into the submandibular gland of athymic nude mice, mCherry+ salivary gland organoids (SGO) integrated into the gland and differentiated into salivary gland phenotypes, including acinar, ductal and myoepithelial structures. Our findings suggest that SG organoids may hold promise for studying SG development, disease progression and drug screening, as well as to develop cell therapies for salivary gland regeneration.
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Direct links to NCBI, no account and no request form: the whole study as GSE342790_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 16 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1508898 and SRA study SRP724918. Searching any of these in the dataset finder brings you back here.

Study design
8 × BXS0114 - Day vs 8 × WA09 - Day

Supports a between-group comparison across 16 samples.

2 replicated groups read from 16 sample titles; they account for 16 of them. Check it against the sample list below before relying on it.

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