GEO series
Molecular and spatial analysis unveils the functional basis of tertiary lymphoid structures in Sjogren’s syndrome[scRNA-seq]
GSE272409
Homo sapiens
Expression profiling by high throughput sequencing
13 samples
Submitted 2024/07/17
Platform GPL24676
Summary
The key role of tertiary lymphoid structures in autoimmune and non-autoimmune conditions has been recently appreciated. While many of the molecular mechanisms involved in tertiary lymphoid structure formation have been identified, their cellular sources and temporal and spatial relationship remain unknown. Using single-cell RNA-sequencing, spatial transcriptomics and proteomics of minor salivary glands of patients with Sjogren’s disease and Sicca Syndrome, ex-vivo and in vivo functional studies, we construct a cellular and spatial map of key components involved in the formation and function of tertiary lymphoid structures. We confirm the presence of a fibroblast cell state and identify an undescribed pericyte/mural cell state with potential immunological functions. The identification of novel cellular properties associated with these structures and the molecular and functional interactions identified by this analysis provide key therapeutic cues for tertiary lymphoid structures associated conditions in autoimmunity and cancer.
Published in
Nayar S, Turner JD, Asam S et al.
· Nature communications 2025
· PMID 39747819
· doi:10.1038/s41467-024-54686-0
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE272409_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 13 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1136831 and SRA study SRP520454. Searching any of these in the dataset finder brings you back here.
Study design
13 conditions, each sampled once — no replicated groups
Read from 13 sample titles: 13 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
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- GSM8401511 SjD, Patient1, scRNAseq
- GSM8401512 SjD, Patient2, scRNAseq
- GSM8401513 SjD, Patient3, scRNAseq
- GSM8401514 SjD, Patient4, scRNAseq
- GSM8401515 SjD, Patient5, scRNAseq
- GSM8401516 SjD, Patient6, scRNAseq
- GSM8401517 SjD, Patient7, scRNAseq
- GSM8401518 non-SjD Sicca, Patient1, scRNAseq
- GSM8401519 non-SjD Sicca, Patient2, scRNAseq
- GSM8401520 non-SjD Sicca, Patient3, scRNAseq
- GSM8401521 non-SjD Sicca, Patient4, scRNAseq
- GSM8401522 non-SjD Sicca, Patient5, scRNAseq
- GSM8401523 non-SjD Sicca, Patient6, scRNAseq
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