GEO series
Single-cell gene expression profiles of cells from primary colorectal cancer and adjacent normal tissue [scRNA-Seq]
GSE294300
Homo sapiens
Expression profiling by high throughput sequencing
36 samples
Submitted 2026/07/22
Platform GPL18573
Summary
Colorectal cancer (CRC) is the third malignancy worldwide. RAS mutant CRC has a worse prognosis and resistant to immune therapies. Current research on the tumor microenvironment of RAS-mutant CRC remains limited, with few studies systematically characterizing its cellular composition or functional dynamics. A total of 36 clinical surgical samples (tumors and paired adjacent normal tissues) from 18 patients with primary CRC were collected for single-cell transcriptome sequencing.At the same time, each patient underwent genome sequencing, and 18 patients were divided into RAS gene mutation group (n=10) and wild-type group (n=8). Therefore, revealing the functional cell types and mechanism of immune evasion in RAS mutant CRC by scRNA-seq may contribute to discover new therapeutic targets.
Published in
Wang J, He Q, Hu J et al.
· Journal of translational medicine 2026
· PMID 42143353
· doi:10.1186/s12967-026-08232-9
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE294300_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 36 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1248964 and SRA study SRP579713. Searching any of these in the dataset finder brings you back here.
Study design
18 × adjacent normal cells, patient vs 18 × colorectal cancer cells, patient
Supports a between-group comparison across 36 samples.
2 replicated groups read from 36 sample titles; they account for 36 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM8901920 adjacent normal cells, patient_01
- GSM8901921 adjacent normal cells, patient_02
- GSM8901922 adjacent normal cells, patient_03
- GSM8901923 adjacent normal cells, patient_04
- GSM8901924 adjacent normal cells, patient_06
- GSM8901925 adjacent normal cells, patient_07
- GSM8901926 adjacent normal cells, patient_08
- GSM8901927 adjacent normal cells, patient_09
- GSM8901928 adjacent normal cells, patient_10
- GSM8901929 adjacent normal cells, patient_11
- GSM8901930 adjacent normal cells, patient_15
- GSM8901931 adjacent normal cells, patient_16
- GSM8901932 adjacent normal cells, patient_17
- GSM8901933 adjacent normal cells, patient_18
- GSM8901934 adjacent normal cells, patient_19
- GSM8901935 adjacent normal cells, patient_20
- GSM8901936 adjacent normal cells, patient_21
- GSM8901937 adjacent normal cells, patient_23
- GSM8901938 colorectal cancer cells, patient_01
- GSM8901939 colorectal cancer cells, patient_02
- GSM8901940 colorectal cancer cells, patient_03
- GSM8901941 colorectal cancer cells, patient_04
- GSM8901942 colorectal cancer cells, patient_06
- GSM8901943 colorectal cancer cells, patient_07
- GSM8901944 colorectal cancer cells, patient_08
- GSM8901945 colorectal cancer cells, patient_09
- GSM8901946 colorectal cancer cells, patient_10
- GSM8901947 colorectal cancer cells, patient_11
- GSM8901948 colorectal cancer cells, patient_15
- GSM8901949 colorectal cancer cells, patient_16
- GSM8901950 colorectal cancer cells, patient_17
- GSM8901951 colorectal cancer cells, patient_18
- GSM8901952 colorectal cancer cells, patient_19
- GSM8901953 colorectal cancer cells, patient_20
- GSM8901954 colorectal cancer cells, patient_21
- GSM8901955 colorectal cancer cells, patient_23
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