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Single-nucleus multiomic profiling of hippocampus and frontal cortex in a C9orf72 knockout mouse model

GSE333807 Mus musculus Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing 52 samples Submitted 2026/06/03 Platform GPL24247Platform GPL34290
Summary
This project contains single-nucleus multiomic sequencing data generated from hippocampal and frontal cortex tissue collected from wild-type, heterozygous, and C9orf72 knockout mice. Single-nucleus transcriptomic and chromatin accessibility profiles were generated using the 10x Genomics Chromium Single Cell Multiome ATAC + Gene Expression platform to simultaneously measure gene expression and chromatin accessibility within individual nuclei. The dataset was generated to investigate cell type-specific molecular and regulatory alterations associated with reduced or absent C9orf72 function in brain regions relevant to amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Libraries were prepared for paired gene expression and chromatin accessibility assays and sequenced on the Illumina NovaSeq 6000 platform.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE333807_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 52 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1469843 and SRA study SRP703205. Searching any of these in the dataset finder brings you back here.

Study design
40 conditions, each sampled once — no replicated groups

Read from the first 40 of 52 sample titles: 40 distinct titles with little repetition. Check it against the sample list below before relying on it.

Samples in this study

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