GEO series
DGCR8 promotes RNA polymerase II pause release independently of DROSHA [ChIP-seq]
GSE324933
Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
32 samples
Submitted 2026/07/30
Platform GPL24247
Summary
DiGeorge syndrome critical region gene 8 (DGCR8) is best known as the RNA binding subunit of the microprocessor complex required for canonical microRNA (miRNA) biogenesis. Whether DGCR8 also exerts a direct, microprocessor independent function in the RNA polymerase II (Pol II) transcription cycle remains unclear. Here, we combined rapid, acute protein depletion with nascent transcription profiling and uncovered a non-canonical role for DGCR8 in transcriptional control in mouse embryonic stem cells (mESCs). We found that DGCR8 broadly occupies promoters and transcription start sites (TSSs) with limited co occupancy by its catalytic microprocessor partner DROSHA. Acute DGCR8 depletion triggers an immediate, genome wide reduction in nascent RNA synthesis that is not phenocopied by acute DROSHA depletion. Mechanistically, DGCR8 loss decreases Pol II engagement at promoters and induces a pervasive blockade in promoter proximal pause release, indicating a direct requirement for DGCR8 in the transition to productive elongation. We further identified physical interactions linking DGCR8 to Enhancer of Rudimentary Homolog (ERH) and the elongation factor SPT5, and showed that acute ERH depletion similarly suppresses nascent transcription. Together, our data implicate a potential model in which promoter bound DGCR8 facilitates Pol II pause release through an ERH–SPT5 linked regulatory network, establishing DGCR8 as a non canonical regulator of the core transcription machinery.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE324933_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 32 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1438123 and SRA study SRP684071. Searching any of these in the dataset finder brings you back here.
Study design
14 conditions, mostly in duplicate
mES, dTAG_DGCR8, DMSO, FLAG, ×2
mES, dTAG_DGCR8, dTAG24h, FLAG, ×2
mES, dTAG_DROSHA, DMSO, FLAG, ×2
mES, dTAG_DROSHA, dTAG24h, FLAG, ×2
mES, dTAG_ERH, non_dTAG, SPT5, ×2
mES, dTAG_ERH, dTAG_3h, SPT5, ×2
mES, dTAG_DROSHA, non_dTAG, ERH, ×2
mES, dTAG_DROSHA, non_dTAG, SPT5, ×2
+10 more
Supports a between-group comparison across 28 samples.
14 replicated groups read from 32 sample titles; they account for 28 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9589046 mES, dTAG_DGCR8, DMSO, FLAG, rep1
- GSM9589047 mES, dTAG_DGCR8, dTAG24h, FLAG, rep1
- GSM9589048 mES, dTAG_DROSHA, DMSO, FLAG, rep1
- GSM9589049 mES, dTAG_DROSHA, dTAG24h, FLAG, rep1
- GSM9589050 mES, dTAG_DGCR8, DMSO, FLAG, rep2
- GSM9589051 mES, dTAG_DGCR8, dTAG24h, FLAG, rep2
- GSM9589052 mES, dTAG_DROSHA, DMSO, FLAG, rep2
- GSM9589053 mES, dTAG_DROSHA, dTAG24h, FLAG, rep2
- GSM9589054 mES, dTAG_DGCR8, DMSO, ERH, rep1
- GSM9589055 mES, dTAG_DGCR8, dTAG24h, ERH, rep1
- GSM9589056 mES, dTAG_DROSHA, DMSO, ERH, rep2
- GSM9589057 mES, dTAG_DROSHA, dTAG24h, ERH, rep2
- GSM9881010 mES, dTAG_ERH, non_dTAG, SPT5, rep1
- GSM9881011 mES, dTAG_ERH, dTAG_3h, SPT5, rep1
- GSM9881012 mES, dTAG_ERH, non_dTAG, SPT5, rep2
- GSM9881013 mES, dTAG_ERH, dTAG_3h, SPT5, rep2
- GSM9881014 mES, dTAG_DROSHA, non_dTAG, ERH, rep1
- GSM9881015 mES, dTAG_DROSHA, non_dTAG, ERH, rep2
- GSM9881016 mES, dTAG_DROSHA, non_dTAG, SPT5, rep1
- GSM9881017 mES, dTAG_DROSHA, non_dTAG, SPT5, rep2
- GSM9881018 mES, dTAG_DROSHA, dTAG_3h, ERH, rep1
- GSM9881019 mES, dTAG_DROSHA, dTAG_3h, ERH, rep2
- GSM9881020 mES, dTAG_DROSHA, dTAG_3h, SPT5, rep1
- GSM9881021 mES, dTAG_DROSHA, dTAG_3h, SPT5, rep2
- GSM9881022 mES, dTAG_DGCR8, non_dTAG, ERH, rep1
- GSM9881023 mES, dTAG_DGCR8, non_dTAG, ERH, rep2
- GSM9881024 mES, dTAG_DGCR8, non_dTAG, SPT5, rep1
- GSM9881025 mES, dTAG_DGCR8, non_dTAG, SPT5, rep2
- GSM9881026 mES, dTAG_DGCR8, dTAG_3h, ERH, rep1
- GSM9881027 mES, dTAG_DGCR8, dTAG_3h, ERH, rep2
- GSM9881028 mES, dTAG_DGCR8, dTAG_3h, SPT5, rep1
- GSM9881029 mES, dTAG_DGCR8, dTAG_3h, SPT5, rep2
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