GEO series

BOLL-containing Aggregate Mediates Translational Regulation During Human Oogenesis

GSE307299 Homo sapiens Other; Expression profiling by high throughput sequencing 14 samples Submitted 2026/04/15 Platform GPL24676

Summary

Human oocyte meiosis utilizes a specialized translational control strategy to coordinate meiotic progression, mediated through dynamic regulation of mRNA stores. While germ cell-specific RNA-binding proteins (RBPs) are known to orchestrate this post-transcriptional program, the mechanistic basis of RBP-mediated cell fate specification remains elusive. Here, we demonstrate that BOLL, a Deleted in Azoospermia (DAZ) family protein, forms protein aggregates during meiotic prophase to drive translational reprogramming in human oogenesis. We determined that BOLL enhance translation efficiency of cell cycle regulators, as demonstrated by integrative translatome-transcriptome analysis combined with RNA immunoprecipitation sequencing. We also revealed the functional interaction network of BOLL with core translation machinery components through its conserved DAZ containing domain. Crucially, we identified the SDS-resistant protein aggregates as BOLL structural signature in human oocyte-like cells, demonstrated by semi-denaturing electrophoretic analysis. These finding delineate a protein aggregate-mediated regulatory paradigm where BOLL aggregates exert spatiotemporal control over cell cycle related genes during the meiotic prophase of human oogenesis.

Published in

BOLL-Containing Aggregates Mediate the Translational Regulation During Human Oogenesis

Li Y, Mao L, Liang B et al. · Cell proliferation 2026 · PMID 41742712 · doi:10.1111/cpr.70181

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Direct links to NCBI, no account and no request form: the whole study as GSE307299_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 14 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1315168 and SRA study SRP617640. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41742712) ↗

Study design

7 conditions, mostly in duplicate

Ribo_BOLL ×2 Ribo_Control ×2 RNA_BOLL ×2 RNA_Control ×2 BOLL ×2 IgG ×2 Input ×2

Supports a between-group comparison across 14 samples.

7 replicated groups read from 14 sample titles; they account for 14 of them. Check it against the sample list below before relying on it.

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