GEO series
Single-Cell RNA-Seq Reveals Immunosuppressive Effects of Triple-Negative Breast Cancer–Derived Exosomes on NK Cell Responses
GSE303346
Homo sapiens
Expression profiling by high throughput sequencing
16 samples
Submitted 2026/07/25
Platform GPL34284
Summary
Triple-negative breast cancer (TNBC) is a highly aggressive and immunogenic subtype that lacks effective targeted therapies. Although tumor-derived exosomes are known to influence immune responses, their direct role in shaping human NK cell plasticity remains insufficiently characterized. In this study, we performed an integrative single-cell multiomic analysis of primary human NK cells following exposure to exosomes isolated from 17 genomically distinct TNBC cell lines. By combining single-cell transcriptomics, we identified conserved and subtype-specific immunoregulatory programs elicited by TNBC-derived exosomes.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE303346_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 16 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1294879 and SRA study SRP602850. Searching any of these in the dataset finder brings you back here.
Study design
8 × Gene expression of NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well vs 8 × ADT expression NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well
Supports a between-group comparison across 16 samples.
2 replicated groups read from 16 sample titles; they account for 16 of them. Check it against the sample list below before relying on it.
Samples in this study
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- GSM9123911 Gene expression of NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well1
- GSM9123912 Gene expression of NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well2
- GSM9123913 Gene expression of NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well3
- GSM9123914 Gene expression of NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well4
- GSM9123915 Gene expression of NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well5
- GSM9123916 Gene expression of NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well6
- GSM9123917 Gene expression of NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well7
- GSM9123918 Gene expression of NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well8
- GSM9123919 ADT expression NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well1
- GSM9123920 ADT expression NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well2
- GSM9123921 ADT expression NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well3
- GSM9123922 ADT expression NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well4
- GSM9123923 ADT expression NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well5
- GSM9123924 ADT expression NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well6
- GSM9123925 ADT expression NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well7
- GSM9123926 ADT expression NK cells samples treated by TNBC-exosomes and one untreated NK cells sample, Well8
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