GEO series
SIRT6 Overexpression Counteracts Chromatin Aging [ATAC-Seq_tg]
GSE294103
Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
28 samples
Submitted 2026/04/12
Platform GPL24247
Summary
Aging is associated with detrimental changes in chromatin structure and gene expression, contributing to inflammation, metabolic decline and tissue dysfunction. SIRT6, a histone deacetylase, plays a key role in maintaining chromatin integrity and promoting longevity. Here, we show that aging leads to increased chromatin accessibility in the murine liver, accompanied by upregulation of inflammation and downregulation of metabolic pathways. Remarkably, SIRT6 overexpression reversed these changes, reducing inflammation and enhancing metabolic function. Notably, ETS family members were enriched in regions with increased accessibility during aging, while liver-enriched transcription factors (LETFs) were enriched in regions with reduced accessibility. ChIP-seq analyses of H3K9ac and H3K56ac binding showed that H3K9ac, but not H3K56ac, is associated with increased accessibility during aging, and that SIRT6 can reverse this effect. Furthermore, AAV-mediated SIRT6 overexpression in aged mice demonstrated that SIRT6 not only slows age-related chromatin changes but can also reverse them, rejuvenating chromatin accessibility to a youthful state.
Published in
Nagar R, Schwartz Z, Katz A et al.
· Nature communications 2026
· PMID 42135312
· doi:10.1038/s41467-026-73115-y
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE294103_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 28 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1248381 and SRA study SRP577387. Searching any of these in the dataset finder brings you back here.
Study design
8 × atacseq_tg, young_wt vs 8 × atacseq_tg, old_wt vs 6 × atacseq_tg, young_sirt6_tg vs 6 × atacseq_tg, old_sirt6_tg
Supports a between-group comparison across 28 samples.
4 replicated groups read from 28 sample titles; they account for 28 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM8898686 atacseq_tg, young_wt_1
- GSM8898687 atacseq_tg, young_wt_2
- GSM8898688 atacseq_tg, young_wt_3
- GSM8898689 atacseq_tg, young_wt_4
- GSM8898690 atacseq_tg, young_wt_5
- GSM8898691 atacseq_tg, young_wt_6
- GSM8898692 atacseq_tg, young_wt_7
- GSM8898693 atacseq_tg, young_wt_8
- GSM8898694 atacseq_tg, young_sirt6_tg_9
- GSM8898695 atacseq_tg, young_sirt6_tg_10
- GSM8898696 atacseq_tg, young_sirt6_tg_11
- GSM8898697 atacseq_tg, young_sirt6_tg_12
- GSM8898698 atacseq_tg, young_sirt6_tg_13
- GSM8898699 atacseq_tg, young_sirt6_tg_14
- GSM8898700 atacseq_tg, old_wt_15
- GSM8898701 atacseq_tg, old_wt_16
- GSM8898702 atacseq_tg, old_wt_17
- GSM8898703 atacseq_tg, old_wt_18
- GSM8898704 atacseq_tg, old_wt_19
- GSM8898705 atacseq_tg, old_wt_20
- GSM8898706 atacseq_tg, old_wt_21
- GSM8898707 atacseq_tg, old_wt_22
- GSM8898708 atacseq_tg, old_sirt6_tg_23
- GSM8898709 atacseq_tg, old_sirt6_tg_24
- GSM8898710 atacseq_tg, old_sirt6_tg_25
- GSM8898711 atacseq_tg, old_sirt6_tg_26
- GSM8898712 atacseq_tg, old_sirt6_tg_27
- GSM8898713 atacseq_tg, old_sirt6_tg_28
Similar datasets
- GSE294085 SIRT6 Overexpression Counteracts Chromatin Aging [ChIP-Seq] 74 samples
- GSE283906 RXR-Mediated Remodeling of Transcriptional and Chromatin Landscapes in APP Mouse Brain: Insights from Integrated Single-Cell RNA and ATAC Profiling 10 samples
- GSE342565 Chromatin accessibility profiling of high endothelial cells (HECs) from mouse peripheral lymph node and Peyer's patch by ATAC-seq 2 samples
- GSE331488 A global view of cell-type-specific temporal dynamics in mammalian aging [I] 771 samples
- GSE331489 A global view of cell-type-specific temporal dynamics in mammalian aging [II] 725 samples
- GSE304211 H4K16ac contributes to chromatin compartment reorganization during mitotic and meiotic transitions [CUTnTAG] 128 samples
- GSE272524 Chromatin-dependent motif syntax defines differentiation trajectories [ChIP-seq] 76 samples
- GSE279088 Molecular dynamics driving phenotypic divergence among KRAS mutants in pancreatic tumorigenesis [ATAC-Seq] 74 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.