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A longitudinal single-cell and spatial multiomic atlas of pediatric high-grade glioma

GSE292623 Homo sapiens Expression profiling by high throughput sequencing 18 samples Submitted 2026/03/11 Platform GPL24676
Summary
Pediatric high-grade glioma (pHGG) is an incurable central nervous system malignancy that is a leading cause of pediatric cancer death. While pHGG shares many similarities to adult glioma, it comprises distinct disease entities. In this study, we longitudinally profile a molecularly diverse cohort of 16 pHGG patients through single-nucleus RNA and ATAC sequencing, whole-genome sequencing, and CODEX spatial proteomics to capture the evolution of neoplastic and microenvironmental features during disease progression and treatment. We define a set of core pHGG neoplastic cell states and observe differential tumor-myeloid interactions between malignant cell phenotypes. We find that essential neuromodulators and the interferon response are upregulated post-therapy and validated malignant cell-intrinsic targets. We observe an increase in oligodendrocytes upon progression and that they coordinate spatial motifs with proneural tumor cells. This multiomic atlas of longitudinal pHGG captures features of therapy response and provides a scalable reference for the study of pediatric brain tumors.
Published in
A longitudinal single-cell and spatial multiomic atlas of pediatric high-grade glioma
Sussman JH, Oldridge DA, Yu W et al. · Cell reports. Medicine 2026 · PMID 42030938 · doi:10.1016/j.xcrm.2026.102766
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Direct links to NCBI, no account and no request form: the whole study as GSE292623_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 18 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1240321 and SRA study SRP572256. Searching any of these in the dataset finder brings you back here.

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