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Targeting a Galectin-3/EGR1 transcriptional complex inhibits the pro-metastatic signature in TRIM49-deficient gastric adenocarcinoma

GSE289465 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing 18 samples Submitted 2025/09/17 Platform GPL20301Platform GPL23227
Summary
Distal metastasis, driven by intracellular signaling rewiring, is a leading cause of cancer mortality. The role of post-translational modifications in metastasis remains unclear. A CRISPR screen in a gastric adenocarcinoma (GAC) mouse model identified TRIM49 as a metastasis suppressor. TRIM49 is downregulated in most advanced GACs, correlating with poor prognosis. Galectin-3, stabilized in TRIM49-deficient cells due to impaired degradation, forms a complex with EGR1, enhancing pro-metastatic gene expression. Disrupting this complex with GB1107 inhibits metastasis in mouse models. The Galectin-3/EGR1 complex in TRIM49-deficient GAC is a key driver of metastasis and a potential therapeutic target.
Published in
TRIM49 Deficiency Stabilizes a Galectin-3/EGR1 Transcriptional Complex That Drives Invasiveness of Gastric Adenocarcinoma
Qin ZY, Che LR, Tian S et al. · Cancer research 2026 · PMID 40997315 · doi:10.1158/0008-5472.CAN-25-0252
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Direct links to NCBI, no account and no request form: the whole study as GSE289465_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 18 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1223098 and SRA study SRP563592. Searching any of these in the dataset finder brings you back here.

Study design
7 conditions, mostly in triplicate
Wild type of NuGc3 replication ×3 Gac3 ihibition of NuGc3 replication ×3 WT of NuGc3 replication ×3 over expression TRIM49 of NuGc3 replication ×3 Cut&Tag_NuGC3-Gac3-replication ×2 Cut&Tag_NuGC3-EGR1-replication ×2 Cut&Tag_NuGC3-IgG-replication ×2

Supports a between-group comparison across 18 samples.

7 replicated groups read from 18 sample titles; they account for 18 of them. Check it against the sample list below before relying on it.

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