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Characterization of E2F8-mediated transcriptional regulation in lethal prostate cancer

GSE275631 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing 39 samples Submitted 2025/01/10 Platform GPL24676
Summary
To identify the genomic bindings and transcriptional targets of E2F8 in lethal prostate cancer cells. To examine the potential off-target effects by CRISPR/CasRx-mediated E2F8 knockdown in 293FT cells. To analyze differentially expressed genes upon TT3 LLN-CRISPR/CasRx-mediated E2F8 knockdown in DU145 xenografts.
Published in
Integrative analysis identifies the atypical repressor E2F8 as a targetable transcriptional activator driving lethal prostate cancer
Huang F, Li K, Chen Z et al. · Oncogene 2025 · PMID 39613933 · doi:10.1038/s41388-024-03239-2
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE275631_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 39 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1152279 and SRA study SRP528595. Searching any of these in the dataset finder brings you back here.

Study design
8 conditions, mostly in triplicate
DU145 xeno_TT3 LLN-CasRx/pre-gCtrl ×6 PC3_IgG ChIP ×3 PC3_E2F8 ChIP ×3 DU145_IgG ChIP ×3 DU145_E2F8 ChIP ×3 293FT_pre-gCtrl ×3 293FT_pre-gE2F8 ×3 DU145 xeno_TT3 LLN-CasRx/pre-gE2F8 ×3 +12 more

Supports a between-group comparison across 27 samples.

8 replicated groups read from 39 sample titles; they account for 27 of them. Check it against the sample list below before relying on it.

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