← BioTransfer GEO Dataset Finder
GEO series

CITEseq analysis of human CSF cells in Multiple sclerosis

GSE286068 Homo sapiens Expression profiling by high throughput sequencing; Other 26 samples Submitted 2025/02/01 Platform GPL11154
Summary
In CSF bulk RNA sequence, patients with MS (pwMS) exhibited higher T cell receprot (TCR) clonality and specificity to EBV lytic proteins compared to controls. TCRs from pwMS showed greater clustering coefficient (i.e. TCR similarity) in complementarity-determining regions 3 (CDR3), particularly those predicted to target EBV antigens. Importantly, TCR clustering coefficient was associated with the expression of cytotoxic CD8 module genes in WGCNA, such as CD8A, GZMH, GZMK, and NKG7 and with interferon signaling module. scRNA-seq revealed a subpopulation of GZMK+GZMH+ double positive (DP) CD8 T cells expressing genes related to identified cytotoxicity and interferon signaling modules. These DP CD8 T cells had significantly higher predicted specificity for EBV proteins than other CD8 cells.
Published in
Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines
Parween F, Singh SP, Kathuria N et al. · Nature communications 2025 · PMID 40000641 · doi:10.1038/s41467-025-57002-6
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE286068_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 26 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1207574 and SRA study SRP555878. Searching any of these in the dataset finder brings you back here.

Study design
11 × multiplexed CSF cells vs 5 × CSF cells, PP-MS vs 4 × CSF cells, HD vs 4 × CSF cells, RR-MS

Supports a between-group comparison across 24 samples.

4 replicated groups read from 26 sample titles; they account for 24 of them. Check it against the sample list below before relying on it.

Samples in this study
Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.