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C18orf21/RMP24 and Nepro/RMP64 are RNase MRP-specific subunits required for 40S ribosome biogenesis

GSE279079 Homo sapiens Other; Expression profiling by high throughput sequencing 40 samples Submitted 2025/08/11 Platform GPL34295
Summary
RNase P and RNase MRP are evolutionarily-related ribonucleoprotein complexes that share common protein subunits, but carry out distinct site-specific endonuclease activities. Here, we identify two RNase MRP-specific proteins C18orf21 (RMP24) and Nepro (RMP64). C18orf21 contains a structurally similar N-terminal domain to Rpp21, but has a distinct subcellular localization and RNA-binding specificity, allowing C18orf21 to uniquely associate with RNase MRP. We ectopically express C18orf21 (RNase MRP specific), Nepro (RNase MRP specific), Rpp21 (RNase P specific), and Rpp14 (shared subunit), with a C-terminal GFP and performed RIP-seq to identify bound RNAs. Our data provide a model to understand C18orf21 physical interactions along with its role in ribosomal RNA processing. In addition to this, we knock out C18orf21, Rpp21, and Rpp14 and analyze their transcriptome. We additionally analyze rRNA intermediates in knockout cells.
Published in
RNase MRP subunit composition and role in 40S ribosome biogenesis
Smith EM, Ly J, Haug S et al. · Nature structural & molecular biology 2026 · PMID 41136609 · doi:10.1038/s41594-025-01690-7
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Also filed as BioProject PRJNA1170312 and SRA study SRP537214. Searching any of these in the dataset finder brings you back here.

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