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A conserved HAND2-BMP5-SMAD1/5/9 axis drives hepatic stellate cell activation and extracellular matrix overproduction in multiple fibrotic etiologies

GSE341139 Homo sapiens Expression profiling by high throughput sequencing 10 samples Submitted 2026/08/08 Platform GPL24676
Summary
Hepatic stellate cell (HSC) activation is a key driver of liver fibrogenesis, but the transcriptional response to bone morphogenetic protein 5 (BMP5) in these cells has not been systematically characterized. To address this, we performed RNA‑sequencing on the human LX‑2 hepatic stellate cell line following stimulation with recombinant BMP5 protein, compared with vehicle‑treated controls. Each condition included five independent biological replicates to ensure statistical robustness. Total RNA was extracted, and strand‑specific libraries were prepared for high‑throughput sequencing. This dataset enables the genome‑wide identification of differentially expressed genes, pathway enrichment analyses, and the delineation of BMP5‑regulated networks in HSCs. Our transcriptomic profiles provide a comprehensive resource for understanding the molecular actions of BMP5 in hepatic fibrosis and may reveal potential targets for therapeutic intervention.
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Direct links to NCBI, no account and no request form: the whole study as GSE341139_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 10 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1499629 and SRA study SRP720651. Searching any of these in the dataset finder brings you back here.

Study design
5 × Control vs 5 × BMP5

Supports a between-group comparison across 10 samples.

2 replicated groups read from 10 sample titles; they account for 10 of them. Check it against the sample list below before relying on it.

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